PEDV ORF3 encodes an ion channel protein and regulates virus production

Kai Wang; Wei Lu; Jianfei Chen; Shiqi Xie; Hongyan Shi; Haojen Hsu; Wenjing Yu; Ke Xu; Chao Bian; Wolfgang B. Fischer; Wolfgang Schwarz; Li Feng; Bing Sun

doi:10.1016/j.febslet.2012.01.005

PEDV ORF3 encodes an ion channel protein and regulates virus production

Kai Wang, Wei Lu, Jianfei Chen, Shiqi Xie, Hongyan Shi, Haojen Hsu, Wenjing Yu, Ke Xu, Chao Bian, Wolfgang B. Fischer, Wolfgang Schwarz, Li Feng, Bing Sun^*

^*此作品的通信作者

生醫光電研究所

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140 引文斯高帕斯（Scopus）

摘要

Several studies suggest that the open reading frame 3 (ORF3) gene of porcine epidemic diarrhea virus (PEDV) is related to viral infectivity and pathogenicity, but its function remains unknown. Here, we propose a structure model of the ORF3 protein consisting of four TM domains and forming a tetrameric assembly. ORF3 protein can be detected in PEDV-infected cells and it functions as an ion channel in both Xenopus laevis oocytes and yeast. Mutation analysis showed that Tyr170 in TM4 is important for potassium channel activity. Furthermore, viral production is reduced in infected Vero cells when ORF3 gene is silenced by siRNA. Interestingly, the ORF3 gene from an attenuated PEDV encodes a truncated protein with 49 nucleotide deletions, which lacks the ion channel activity.

原文	English
頁（從 - 到）	384-391
頁數	8
期刊	FEBS Letters
卷	586
發行號	4
DOIs	https://doi.org/10.1016/j.febslet.2012.01.005
出版狀態	Published - 17 2月 2012

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@article{0cf99248045b41de89d7e8d50f6baf81,

title = "PEDV ORF3 encodes an ion channel protein and regulates virus production",

abstract = "Several studies suggest that the open reading frame 3 (ORF3) gene of porcine epidemic diarrhea virus (PEDV) is related to viral infectivity and pathogenicity, but its function remains unknown. Here, we propose a structure model of the ORF3 protein consisting of four TM domains and forming a tetrameric assembly. ORF3 protein can be detected in PEDV-infected cells and it functions as an ion channel in both Xenopus laevis oocytes and yeast. Mutation analysis showed that Tyr170 in TM4 is important for potassium channel activity. Furthermore, viral production is reduced in infected Vero cells when ORF3 gene is silenced by siRNA. Interestingly, the ORF3 gene from an attenuated PEDV encodes a truncated protein with 49 nucleotide deletions, which lacks the ion channel activity.",

keywords = "Ion channel, Molecular dynamics, ORF3, PEDV",

author = "Kai Wang and Wei Lu and Jianfei Chen and Shiqi Xie and Hongyan Shi and Haojen Hsu and Wenjing Yu and Ke Xu and Chao Bian and Fischer, {Wolfgang B.} and Wolfgang Schwarz and Li Feng and Bing Sun",

note = "Funding Information: We thank Deubel Vincent and Ben Bravery for reviewing the manuscript. Richard F. Gaber kindly supplied the K + uptake-deficient yeast strain. This work was supported by grants from CAS ( KSCX2-YW-R-161 , KSCX2-YW-R-169 , KSCX2-EW-J-14 ), National Ministry of Science and Technology ( 2007DFC31700 ), National Natural Science Foundation of China ( 30950002 , 30623003 , 30721065 , 30801011 , 30870126 , 90713044 ), National Science and Technology Major Project (2009ZX10004-016, 2008ZX10004-002, 2009ZX10004-105), National 973 key project (2007CB512404), grants from SPHRF ( SPHRF2008001 , SPHRF2009001 ), National 863 project (2006AA02A247), and Li Kha Shing Foundation.",

year = "2012",

month = feb,

day = "17",

doi = "10.1016/j.febslet.2012.01.005",

language = "English",

volume = "586",

pages = "384--391",

journal = "FEBS Letters",

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T1 - PEDV ORF3 encodes an ion channel protein and regulates virus production

AU - Wang, Kai

AU - Lu, Wei

AU - Chen, Jianfei

AU - Xie, Shiqi

AU - Shi, Hongyan

AU - Hsu, Haojen

AU - Yu, Wenjing

AU - Xu, Ke

AU - Bian, Chao

AU - Fischer, Wolfgang B.

AU - Schwarz, Wolfgang

AU - Feng, Li

AU - Sun, Bing

N1 - Funding Information: We thank Deubel Vincent and Ben Bravery for reviewing the manuscript. Richard F. Gaber kindly supplied the K + uptake-deficient yeast strain. This work was supported by grants from CAS ( KSCX2-YW-R-161 , KSCX2-YW-R-169 , KSCX2-EW-J-14 ), National Ministry of Science and Technology ( 2007DFC31700 ), National Natural Science Foundation of China ( 30950002 , 30623003 , 30721065 , 30801011 , 30870126 , 90713044 ), National Science and Technology Major Project (2009ZX10004-016, 2008ZX10004-002, 2009ZX10004-105), National 973 key project (2007CB512404), grants from SPHRF ( SPHRF2008001 , SPHRF2009001 ), National 863 project (2006AA02A247), and Li Kha Shing Foundation.

PY - 2012/2/17

Y1 - 2012/2/17

N2 - Several studies suggest that the open reading frame 3 (ORF3) gene of porcine epidemic diarrhea virus (PEDV) is related to viral infectivity and pathogenicity, but its function remains unknown. Here, we propose a structure model of the ORF3 protein consisting of four TM domains and forming a tetrameric assembly. ORF3 protein can be detected in PEDV-infected cells and it functions as an ion channel in both Xenopus laevis oocytes and yeast. Mutation analysis showed that Tyr170 in TM4 is important for potassium channel activity. Furthermore, viral production is reduced in infected Vero cells when ORF3 gene is silenced by siRNA. Interestingly, the ORF3 gene from an attenuated PEDV encodes a truncated protein with 49 nucleotide deletions, which lacks the ion channel activity.

AB - Several studies suggest that the open reading frame 3 (ORF3) gene of porcine epidemic diarrhea virus (PEDV) is related to viral infectivity and pathogenicity, but its function remains unknown. Here, we propose a structure model of the ORF3 protein consisting of four TM domains and forming a tetrameric assembly. ORF3 protein can be detected in PEDV-infected cells and it functions as an ion channel in both Xenopus laevis oocytes and yeast. Mutation analysis showed that Tyr170 in TM4 is important for potassium channel activity. Furthermore, viral production is reduced in infected Vero cells when ORF3 gene is silenced by siRNA. Interestingly, the ORF3 gene from an attenuated PEDV encodes a truncated protein with 49 nucleotide deletions, which lacks the ion channel activity.

KW - Ion channel

KW - Molecular dynamics

KW - ORF3

KW - PEDV

UR - http://www.scopus.com/inward/record.url?scp=84862831888&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2012.01.005

DO - 10.1016/j.febslet.2012.01.005

M3 - Article

C2 - 22245155

AN - SCOPUS:84862831888

SN - 0014-5793

VL - 586

SP - 384

EP - 391

JO - FEBS Letters

JF - FEBS Letters

IS - 4

ER -

PEDV ORF3 encodes an ion channel protein and regulates virus production

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