PEDV ORF3 encodes an ion channel protein and regulates virus production

Kai Wang, Wei Lu, Jianfei Chen, Shiqi Xie, Hongyan Shi, Haojen Hsu, Wenjing Yu, Ke Xu, Chao Bian, Wolfgang B. Fischer, Wolfgang Schwarz, Li Feng, Bing Sun*

*此作品的通信作者

研究成果: Article同行評審

114 引文 斯高帕斯(Scopus)

摘要

Several studies suggest that the open reading frame 3 (ORF3) gene of porcine epidemic diarrhea virus (PEDV) is related to viral infectivity and pathogenicity, but its function remains unknown. Here, we propose a structure model of the ORF3 protein consisting of four TM domains and forming a tetrameric assembly. ORF3 protein can be detected in PEDV-infected cells and it functions as an ion channel in both Xenopus laevis oocytes and yeast. Mutation analysis showed that Tyr170 in TM4 is important for potassium channel activity. Furthermore, viral production is reduced in infected Vero cells when ORF3 gene is silenced by siRNA. Interestingly, the ORF3 gene from an attenuated PEDV encodes a truncated protein with 49 nucleotide deletions, which lacks the ion channel activity.

原文English
頁(從 - 到)384-391
頁數8
期刊FEBS Letters
586
發行號4
DOIs
出版狀態Published - 17 2月 2012

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