Pathogenic autoantibodies to IFN-γ act through the impedance of receptor assembly and Fc-mediated response

Han Po Shih, Jing Ya Ding, Junel Sotolongo Bellón, Yu Fang Lo, Pei Han Chung, He Ting Ting, Jhan Jie Peng, Tsai Yi Wu, Chia Hao Lin, Chia Chi Lo, You Ning Lin, Chun Fu Yeh, Jiun Bo Chen, Ting Shu Wu, Yuag Meng Liu, Chen Yen Kuo, Shang Yu Wang, Kun Hua Tu, Chau Yee Ng, Wei Te LeiYu Huan Tsai, Jou Han Chen, Ya Ting Chuang, Jing Yi Huang, Félix A. Rey, Hung Kai Chen, Tse Wen Chang, Jacob Piehler, Chih Yu Chi*, Cheng Lung Ku*

*此作品的通信作者

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

Anti-interferon (IFN)–γ autoantibodies (AIGAs) are a pathogenic factor in late-onset immunodeficiency with disseminated mycobacterial and other opportunistic infections. AIGAs block IFN-γ function, but their effects on IFN-γ signaling are unknown. Using a single-cell capture method, we isolated 19 IFN-γ–reactive monoclonal antibodies (mAbs) from patients with AIGAs. All displayed high-affinity (KD < 10−9 M) binding to IFN-γ, but only eight neutralized IFN-γ–STAT1 signaling and HLA-DR expression. Signal blockade and binding affinity were correlated and attributed to somatic hypermutations. Cross-competition assays identified three nonoverlapping binding sites (I–III) for AIGAs on IFN-γ. We found that site I mAb neutralized IFN-γ by blocking its binding to IFN-γR1. Site II and III mAbs bound the receptor-bound IFN-γ on the cell surface, abolishing IFN-γR1–IFN-γR2 heterodimerization and preventing downstream signaling. Site III mAbs mediated antibody-dependent cellular cytotoxicity, probably through antibody–IFN-γ complexes on cells. Pathogenic AIGAs underlie mycobacterial infections by the dual blockade of IFN-γ signaling and by eliminating IFN-γ–responsive cells.

原文English
文章編號e20212126
期刊Journal of Experimental Medicine
219
發行號9
DOIs
出版狀態Published - 2022

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