Oxygen plasma immersion ion implantation treatment enhances the human bone marrow mesenchymal stem cells responses to titanium surface for dental implant application

Chih Hsiung Yang, Yu Chen Li, Wen Fa Tsai, Chi Fong Ai, Her Hsiung Huang*

*此作品的通信作者

研究成果: Article同行評審

31 引文 斯高帕斯(Scopus)

摘要

Objectives: The present investigation utilized a novel oxygen plasma immersion ion implantation (O-PIII) treatment to create a dense and thin oxide layer on a titanium (Ti) surface for dental implant application. Materials and methods: This study evaluated the behavior of human bone marrow mesenchymal stem cells (hMSCs) on O-PIII-treated Ti. The O-PIII treatments were performed using different oxygen ion doses (TL: 1 × 1016; TM: 4 × 1016; TH: 1 × 1017 ions/cm2). Results: Analysis using an X-ray photoelectron spectrometer (XPS) and high resolution X-ray diffractometer (HR-XRD) indicated that the O-PIII-treated specimen TM had the highest proportion of rutile phase TiO2 component. The O-PIII-treated specimen TM had the greatest protein adsorption capability of the test Ti surfaces using XPS analysis and bicinchoninic acid (BCA) protein assay. Immunofluorescent staining revealed that hMSCs had the best cell adhesion on the O-PIII-treated specimen TM, whereas green fluorescent protein (GFP)-labeled hMSCs experienced the fastest cell migration based on a wound healing assay. Other assays, including MTT assay, Alizarin red S staining and Western blot analysis, demonstrated that the adhered hMSCs exhibited the greatest cell proliferation, mineralization, and differentiation capabilities on the TM specimen. Conclusions: Oxidated Ti (primarily rutile TiO2) was produced using a facile and rapid O-PIII treatment procedure, which enhances the biocompatibility of the Ti surface with potential implications for further dental implant application.

原文English
頁(從 - 到)166-175
頁數10
期刊Clinical Oral Implants Research
26
發行號2
DOIs
出版狀態Published - 1 2月 2015

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