Overexpression of SmeDEF efflux pump decreases aminoglycoside resistance in Stenotrophomonas maltophilia

Yi Wei Huang; Cheng Wen Lin; Hsiao Chen Ning; Yi Tsung Lin; Yi Chih Chang; Tsuey Ching Yang

doi:10.1128/AAC.02685-16

Overexpression of SmeDEF efflux pump decreases aminoglycoside resistance in Stenotrophomonas maltophilia

Yi Wei Huang, Cheng Wen Lin, Hsiao Chen Ning, Yi Tsung Lin, Yi Chih Chang^*, Tsuey Ching Yang

^*此作品的通信作者

研究成果: Article › 同行評審

10 引文斯高帕斯（Scopus）

摘要

The SmeDEF pump of Stenotrophomonas maltophilia is negatively regulated by SmeT. In this study, strains KJΔT (smeT deletion mutant) and KJT-Dm (mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/ nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, in either KJΔT or KJT-Dm, downregulated smeYZ expression, which is responsible for the reduced aminoglycoside resistance. Furthermore, the SmeRySy two-component regulatory system was downregulated in response to SmeDEF overexpression, which supports its involvement in the regulatory circuit.

原文	English
文章編號	e02685
期刊	Antimicrobial Agents and Chemotherapy
卷	61
發行號	5
DOIs	https://doi.org/10.1128/AAC.02685-16
出版狀態	Published - 5月 2017

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10.1128/AAC.02685-16

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title = "Overexpression of SmeDEF efflux pump decreases aminoglycoside resistance in Stenotrophomonas maltophilia",

abstract = "The SmeDEF pump of Stenotrophomonas maltophilia is negatively regulated by SmeT. In this study, strains KJΔT (smeT deletion mutant) and KJT-Dm (mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/ nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, in either KJΔT or KJT-Dm, downregulated smeYZ expression, which is responsible for the reduced aminoglycoside resistance. Furthermore, the SmeRySy two-component regulatory system was downregulated in response to SmeDEF overexpression, which supports its involvement in the regulatory circuit.",

keywords = "Antibiotic resistance, Bacteria, Efflux pump, Two-component regulatory system",

author = "Huang, {Yi Wei} and Lin, {Cheng Wen} and Ning, {Hsiao Chen} and Lin, {Yi Tsung} and Chang, {Yi Chih} and Yang, {Tsuey Ching}",

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doi = "10.1128/AAC.02685-16",

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T1 - Overexpression of SmeDEF efflux pump decreases aminoglycoside resistance in Stenotrophomonas maltophilia

AU - Huang, Yi Wei

AU - Lin, Cheng Wen

AU - Ning, Hsiao Chen

AU - Lin, Yi Tsung

AU - Chang, Yi Chih

AU - Yang, Tsuey Ching

PY - 2017/5

Y1 - 2017/5

N2 - The SmeDEF pump of Stenotrophomonas maltophilia is negatively regulated by SmeT. In this study, strains KJΔT (smeT deletion mutant) and KJT-Dm (mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/ nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, in either KJΔT or KJT-Dm, downregulated smeYZ expression, which is responsible for the reduced aminoglycoside resistance. Furthermore, the SmeRySy two-component regulatory system was downregulated in response to SmeDEF overexpression, which supports its involvement in the regulatory circuit.

AB - The SmeDEF pump of Stenotrophomonas maltophilia is negatively regulated by SmeT. In this study, strains KJΔT (smeT deletion mutant) and KJT-Dm (mutant with a defective SmeT-binding site) showed increased resistance to chloramphenicol/ nalidixic acid/macrolides and susceptibility to aminoglycoside. Overexpression of the SmeDEF pump, in either KJΔT or KJT-Dm, downregulated smeYZ expression, which is responsible for the reduced aminoglycoside resistance. Furthermore, the SmeRySy two-component regulatory system was downregulated in response to SmeDEF overexpression, which supports its involvement in the regulatory circuit.

KW - Antibiotic resistance

KW - Bacteria

KW - Efflux pump

KW - Two-component regulatory system

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U2 - 10.1128/AAC.02685-16

DO - 10.1128/AAC.02685-16

M3 - Article

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AN - SCOPUS:85018171146

SN - 0066-4804

VL - 61

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 5

M1 - e02685

ER -

Overexpression of SmeDEF efflux pump decreases aminoglycoside resistance in Stenotrophomonas maltophilia

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