ORF8a of SARS-CoV forms an ion channel: Experiments and molecular dynamics simulations

Cheng Chang Chen, Jens Krüger, Issara Sramala, Hao Jen Hsu, Peter Henklein, Yi Ming Arthur Chen, Wolfgang B. Fischer

研究成果: Article同行評審

41 引文 斯高帕斯(Scopus)

摘要

ORF8a protein is 39 residues long and contains a single transmembrane domain. The protein is synthesized using solid phase peptide synthesis and reconstituted into artificial lipid bilayers that forms cation-selective ion channels with a main conductance level of 8.9 ± 0.8 pS at elevated temperature (38.5 °C). Computational modeling studies including multi nanosecond molecular dynamics simulations in a hydrated POPC lipid bilayer are done with a 22 amino acid transmembrane helix to predict a putative homooligomeric helical bundle model. A structural model of a pentameric bundle is proposed with cysteines, serines and threonines facing the pore.

原文English
頁(從 - 到)572-579
頁數8
期刊Biochimica et Biophysica Acta - Biomembranes
1808
發行號2
DOIs
出版狀態Published - 2月 2011

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