@article{e3fe86cfb16f40e58b35c8ec9d2d83a8,
title = "ORF8a of SARS-CoV forms an ion channel: Experiments and molecular dynamics simulations",
abstract = "ORF8a protein is 39 residues long and contains a single transmembrane domain. The protein is synthesized using solid phase peptide synthesis and reconstituted into artificial lipid bilayers that forms cation-selective ion channels with a main conductance level of 8.9 ± 0.8 pS at elevated temperature (38.5 °C). Computational modeling studies including multi nanosecond molecular dynamics simulations in a hydrated POPC lipid bilayer are done with a 22 amino acid transmembrane helix to predict a putative homooligomeric helical bundle model. A structural model of a pentameric bundle is proposed with cysteines, serines and threonines facing the pore.",
keywords = "Bilayer recording, Ion channel, Molecular dynamics, ORF8a, SARS-CoV",
author = "Chen, {Cheng Chang} and Jens Kr{\"u}ger and Issara Sramala and Hsu, {Hao Jen} and Peter Henklein and Chen, {Yi Ming Arthur} and Fischer, {Wolfgang B.}",
note = "Funding Information: WBF acknowledges the National Yang-Ming University and the Government of Taiwan for financial support (Aim of Excellence Program), as well as the National Science Council of Taiwan (NSC) . JK acknowledges a fellowship granted jointly by the Alexander von Humboldt-Foundation and NSC .",
year = "2011",
month = feb,
doi = "10.1016/j.bbamem.2010.08.004",
language = "English",
volume = "1808",
pages = "572--579",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "2",
}