Opioid and Antiopioid Actions of Tyr-MIF-1, Tyr-W-MIF-1 and Hemorphin-4 on Rat Locus Coeruleus Neurons: Intracellular Recording in vitro

Yea Ru Yang, Tsai Hsien Chiu*

*此作品的通信作者

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

Intracellular recording was used to study the effects of naturally occurring opioid tetrapeptides, including Tyr-Pro-Leu-Gly-NH 2 (Tyr-MIF-1), Tyr-Pro-Trp-Gly-NH 2 (Tyr-W-MIF-1), and Tyr-Pro-Trp-Thr-OH (hemorphin-4), on neurons of the locus coeruleus (LC) in the rat, in a totally submerged brain slice preparation. These tetrapeptides which, like other opioids, caused inhibitory effects on all LC neurons tested. Tyr-MIF-1 (10-100 μM), Tyr-W-MIF-1 (1-10 μM) and hemorphin-4 (1-30 μM) caused dose-related inhibition of the spontaneous firing. The IC 50 were 37.5, 3.8 and 6.7 μM for Tyr-MIF-1, Tyr-W-MIF-1 and hemorphin-4, respectively. In addition, these tetrapeptides were tested for their blocking effects on the Tyr-D-Arg-Phe-sarcosine-OH (TAPS)-induced inhibitory actions. The results showed that Tyr-MIF-1, Tyr-W-MIF-1 and hemorphin-4 significantly decreased the actions of lower concentration of TAPS (30 nM), but did not decrease the actions of higher concentration of TAPS (100 nM). It is concluded that the blood-derived peptide hemorphin-4 and the endogenous peptides Tyr-W-MIF-1 and Tyr-MIF-1 possess opioid and antiopioid actions in the LC neurons.

原文English
頁(從 - 到)131-135
頁數5
期刊Chinese Journal of Physiology
40
發行號3
出版狀態Published - 1 9月 1997

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