Observation of frequency-domain fluorescence anomalous phase advance due to dark-state hysteresis

Evangelos Gatzogiannis, Xinxin Zhu, Ya-Ting Kao, Wei Min*

*此作品的通信作者

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Frequency-domain fluorescence spectroscopy, commonly referred to as phase fluorometry, is a classic approach to study the lifetime dynamics of fluorescent systems. Here we report an interesting phenomenon: unlike conventional fluorescence lifetime phase fluorometry in which the fluorescence trace always lags behind the modulated excitation source, the detected signal from certain fluorophores can actually exhibit fluorescence anomalous phase advance (FAPA) as if the fluorescence is emitted "ahead"of the source. FAPA is pronounced only within a range of modulation frequencies that are outside quasi-static and quasi-equilibrium conditions. We attribute FAPA to photoinduced dark state hysteresis, supported by both simulations of photodynamic transitions and experiments with dark-state promoters and quenchers. Being a fast and straightforward frequency-domain reporter, FAPA offers a unique and specific contrast mechanism for dark state dynamics sensing and imaging.

原文English
頁(從 - 到)461-466
頁數6
期刊Journal of Physical Chemistry Letters
2
發行號5
DOIs
出版狀態Published - 3 三月 2011

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