NUSAS: Negative pressure driving HEPG2/3T3 cells mixing/gradient co-culture inside U trapper array on rapid multicellular spheroid assembling system

S. M. Yang, C. Y. Fu, S. Y. Tseng, V. P. Srinivasu, S. Shilpa, H. Y. Chang, Long Hsu, C. H. Liu*

*此作品的通信作者

研究成果: Conference contribution同行評審

1 引文 斯高帕斯(Scopus)

摘要

This article presents a Negative-pressure-powered U-trapper of Spheroid Assembling System (NUSAS) and four unique features are addressed: (1) Negative pressure powered medium driving, (2) HepG2/3T3 gradient distribution and mixing co-culture, (3) Cell trapping effect via gravity force and liquid flow co-activation, and (4) Large area of U trapper array. The development of in vitro liver device benefits liver toxicology and metabolism.[1,2] However, due to the complexity of human liver tissue, it has been rarely discussed what kind of hepatocyte to fibroblast ratio has nearest performance to a real liver tissue. Here, we culture liver/fibroblast cells with gradient ratio as multicellular assembling spheroids simultaneously to find this ratio. These results based on our efficient approaches give us meaningful foundations to investigate biomedical character of 3D HepG2/3T3 artificial tissue in different mixing cell conditions.

原文English
主出版物標題2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
頁面1077-1080
頁數4
DOIs
出版狀態Published - 7 5月 2012
事件2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
持續時間: 29 1月 20122 2月 2012

出版系列

名字Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(列印)1084-6999

Conference

Conference2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
國家/地區France
城市Paris
期間29/01/122/02/12

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