Nonmuscle Myosin IIB Is Involved in the Guidance of Fibroplast Migration

Chun Min Lo, Denis B. Buxton, Gregoxy C.H. Chua, Micah Dembo, Robert S. Adelstein, Yu Li Wang*

*此作品的通信作者

研究成果: Article同行評審

205 引文 斯高帕斯(Scopus)

摘要

Although myosin II is known to play an important role in cell migration, little is known about its specific functions. We have addressed the function of one of the isoforms of myosin II, myosin IIB, by analyzing the movement and mechanical characteristics of fibroblasts where this protein has been ablated by gene disruption. Myosin IIB null cells displayed multiple unstable and disorganized protrusions, although they were still able to generate a large fraction of traction forces when cultured on flexible polyacrylamide substrates. However, the traction forces were highly disorganized relative to the direction of cell migration. Analysis of cell migration patterns indicated an increase in speed and decrease in persistence, which were likely responsible for the defects in directional movements as demonstrated with Boyden chambers. In addition, unlike control cells, mutant cells failed to respond to mechanical signals such as compressing forces and changes in substrate rigidity. Immunofluorescence staining indicated that myosin IIB was localized preferentially along stress fibers in the interior region of the cell. Our results suggest that myosin IIB is involved not in propelling but in directing the cell movement, by coordinating protrusive activities and stabilizing the cell polarity.

原文English
頁(從 - 到)982-989
頁數8
期刊Molecular Biology of the Cell
15
發行號3
DOIs
出版狀態Published - 1 3月 2004

指紋

深入研究「Nonmuscle Myosin IIB Is Involved in the Guidance of Fibroplast Migration」主題。共同形成了獨特的指紋。

引用此