Nitric oxide-release study of a bio-inspired copper(i)-nitrito complex under chemical and biological conditions

Wan Jung Chuang, Manmath Narwane, Hsing Yin Chen, Chai Lin Kao, Bin Huang, Kuang Mei Hsu, Yun-Ming Wang*, Sodio C.N. Hsu

*此作品的通信作者

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

The selective and efficient nitrite reduction process is ubiquitous in biological systems. To understand copper-mediated nitrite reduction, we developed a bio-inspired model system to investigate the mechanism of copper-containing nitrite reductase. A well-characterized copper(i)-nitrate complex with amino functionalized 2-(diphenylphosphino)aniline ligands, [(Ph2PC6H4(o-NH2))2Cu(ONO)], demonstrated the aniline protonation will cause NO release in an acidic environment. To further understand NO releasing ability, we also performed pH-dependency experiments and confocal imaging to release NO under physiological buffer conditions. According to titration and spectroscopic studies on the protonation reaction of complex [(Ph2PC6H4(o-NH2))2Cu(ONO)], we proposed a mechanistic pathway for proton transfer and NO release. Furthermore, DFT calculations predicted that the release of NO takes place via aniline in both organic and aqueous media. These results highlight the importance of the proton-rich microenvironment around the copper(i)-nitrite core to induce nitrate reduction in a chemical and biological environment.

原文English
頁(從 - 到)13151-13157
頁數7
期刊Dalton Transactions
47
發行號37
DOIs
出版狀態Published - 8月 2018

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