TY - JOUR
T1 - Neuronal differentiation of human placenta-derived multi-potent stem cells enhanced by cell body oscillation on gelatin hydrogel
AU - Li, Chuan
AU - Liu, Isaac K.K.
AU - Tsao, C. Y.
AU - Chan, Vincent
N1 - Publisher Copyright:
© The Author(s) 2014.
PY - 2014/11/11
Y1 - 2014/11/11
N2 - Gelatin is a biocompatible material commonly employed in biomaterial design and tissue engineering. However, there is currently a lack of research into the development of gelatin hydrogels for facilitating specific lineage development of stem cells. In this study, the neuronal differentiation of human placenta-derived multi-potent (stem) cells was systematically optimized through the engineering of the gelatin hydrogel properties. The swelling ratio of Type A or Type B gelatin hydrogel changes during hydrogel formation in the gelatin concentration ranges from 16 to 6 wt%. In general, placenta-derived multi-potent (stem) cells effectively adhere on both, acidic and basic gelatin hydrogels with different swelling ratios as shown by the high attachment ratio of around 80%. Interestingly, adhered placenta-derived multi-potent (stem) cells had significant cell body oscillations on either 6 or 10 wt% gelatin hydrogels during the first 3 h of cell seeding. For placenta-derived multi-potent (stem) cells pre-cultured on 6 and 10 wt% gelatin hydrogel for either 2 or 12 h and subjected to 3-isobutyl-1-methylxanthine to induce neuronal differentiation, the periodic contraction and extension of placenta-derived multi-potent (stem) cells pre-cultured for 2 h successfully directed the cells into neuron-like lineages. In contrast, the lack of cell body oscillation restrained the placenta-derived multi-potent (stem) cells pre-cultured for 12 h from differentiating into neuronal cells on the same gelatin hydrogels in response to 3-isobutyl-1-methylxanthine stimulation. Overall, the possibility of engineering the properties of gelatin hydrogel to trigger stem cell development into a neuronal lineage through cell body oscillations was clearly demonstrated.
AB - Gelatin is a biocompatible material commonly employed in biomaterial design and tissue engineering. However, there is currently a lack of research into the development of gelatin hydrogels for facilitating specific lineage development of stem cells. In this study, the neuronal differentiation of human placenta-derived multi-potent (stem) cells was systematically optimized through the engineering of the gelatin hydrogel properties. The swelling ratio of Type A or Type B gelatin hydrogel changes during hydrogel formation in the gelatin concentration ranges from 16 to 6 wt%. In general, placenta-derived multi-potent (stem) cells effectively adhere on both, acidic and basic gelatin hydrogels with different swelling ratios as shown by the high attachment ratio of around 80%. Interestingly, adhered placenta-derived multi-potent (stem) cells had significant cell body oscillations on either 6 or 10 wt% gelatin hydrogels during the first 3 h of cell seeding. For placenta-derived multi-potent (stem) cells pre-cultured on 6 and 10 wt% gelatin hydrogel for either 2 or 12 h and subjected to 3-isobutyl-1-methylxanthine to induce neuronal differentiation, the periodic contraction and extension of placenta-derived multi-potent (stem) cells pre-cultured for 2 h successfully directed the cells into neuron-like lineages. In contrast, the lack of cell body oscillation restrained the placenta-derived multi-potent (stem) cells pre-cultured for 12 h from differentiating into neuronal cells on the same gelatin hydrogels in response to 3-isobutyl-1-methylxanthine stimulation. Overall, the possibility of engineering the properties of gelatin hydrogel to trigger stem cell development into a neuronal lineage through cell body oscillations was clearly demonstrated.
KW - Human placenta-derived stem cells
KW - cellular image processing
KW - gelatin hydrogel
KW - neuronal differentiation
UR - http://www.scopus.com/inward/record.url?scp=84908893472&partnerID=8YFLogxK
U2 - 10.1177/0883911514553903
DO - 10.1177/0883911514553903
M3 - Article
AN - SCOPUS:84908893472
SN - 0883-9115
VL - 29
SP - 529
EP - 544
JO - Journal of Bioactive and Compatible Polymers
JF - Journal of Bioactive and Compatible Polymers
IS - 6
ER -