Multiple approaches to characterize the microbial community in a thermophilic anaerobic digester running on swine manure: A case study

Nguyen Ngoc Tuan, Yi Chia Chang, Chang Ping Yu, Shir Ly Huang*

*此作品的通信作者

研究成果: Article同行評審

44 引文 斯高帕斯(Scopus)

摘要

In this study, the first survey of microbial community in thermophilic anaerobic digester using swine manure as sole feedstock was performed by multiple approaches including denaturing gradient gel electrophoresis (DGGE), clone library and pyrosequencing techniques. The integrated analysis of 21 DGGE bands, 126 clones and 8506 pyrosequencing read sequences revealed that Clostridia from the phylum Firmicutes account for the most dominant Bacteria. In addition, our analysis also identified additional taxa that were missed by the previous researches, including members of the bacterial phyla Synergistetes, Planctomycetes, Armatimonadetes, Chloroflexi and Nitrospira which might also play a role in thermophilic anaerobic digester. Most archaeal 16S rRNA sequences could be assigned to the order Methanobacteriales instead of Methanomicrobiales comparing to previous studies. In addition, this study reported that the member of Methanothermobacter genus was firstly found in thermophilic anaerobic digester.

原文English
頁(從 - 到)717-724
頁數8
期刊Microbiological Research
169
發行號9-10
DOIs
出版狀態Published - 9月 2014

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