Multiphoton microscopy in defining liver function

Camilla A. Thorling, Darrell Crawford, Frank J. Burczynski, Xin Liu, Ian Liau, Michael S. Roberts*


研究成果: Review article同行評審

34 引文 斯高帕斯(Scopus)


Multiphoton microscopy is the preferred method when in vivo deep-tissue imaging is required. This review presents the application of multiphoton microscopy in defining liver function. In particular, multiphoton microscopy is useful in imaging intracellular events, such as mitochondrial depolarization and cellular metabolism in terms of NAD(P)H changes with fluorescence lifetime imaging microscopy. The morphology of hepatocytes can be visualized without exogenously administered fluorescent dyes by utilizing their autofluorescence and second harmonic generation signal of collagen, which is useful in diagnosing liver disease. More specific imaging, such as studying drug transport in normal and diseased livers are achievable, but require exogenously administered fluorescent dyes. If these techniques can be translated into clinical use to assess liver function, it would greatly improve early diagnosis of organ viability, fibrosis, and cancer.

期刊Journal of Biomedical Optics
出版狀態Published - 1 9月 2014


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