@article{3f192692ac1846e29ca5bb287c10669e,
title = "Multi-photon fluorescence microscopy - The response of plant cells to high intensity illumination",
abstract = "Multi-photon fluorescence microscopy has been cited for its advantage in increased depth penetration due to low linear absorption and scattering coefficient of biological specimen in the near infrared (NIR) range. Because of the need of high peak power for efficiently exciting two-photon fluorescence, the relationship between cell damage and peak power has become an interesting and much debated topic in the applications of multi-photon fluorescence microscopy. It is conceivable that at high illumination intensity, non-linear photochemical processes have impacts on cell physiology and viability in ways much different from low illumination in the linear domain. In this article, we discuss some of the issues in two-photon fluorescence microscopy, including the degree of transparency of the specimen, a comparison of single- and two-photon excited fluorescence spectra, and the cell damage under high intensity illumination, using plant cells as a model.",
keywords = "Absorption, Arabidopsis, Auto-fluorescence, Cell damage, Plant cell, Protoplast, Scattering, Two-photon fluorescence microscopy",
author = "Cheng, {Ping chin} and Lin, {Bai Ling} and Kao, {Fu Jen} and Min Gu and Xu, {Ming Gun} and Xiaosang Gan and Huang, {Mao Kuo} and Wang, {Yung Shun}",
note = "Funding Information: We are grateful for the research support of Academia Sinica to BLL, the grant support from the Australia Research Council to MG, and the research grants and travel support from the National Science Council, Republic of China, to BLL (NSC-88-2311-B-001-087), PCC (NSC-88-2811-B-001-0023) and FJK (NSC89-2112-M-110-016 and NSC89-2216-E-110-003). We thank Mr Jang-shiun Wang and Mr Rang-Wu Chen at IMB, Academia Sinica, Mr Wen-Jen yang at the Dept. of Biology, NSYSU, and Dr Yunshen Hu at St. Vincent's Institute of Medical Research, Melbourne, for their help in sample preparation. We would also like to thank Drs Chin-Ti Chen and Chien-Chung Cheng at the Institute of Chemistry, Academia Sinica, for kindly providing the spectrophotometer and the fluorometer used in this study. PCC, BLL, FJK and MG contributed equally and therefore are co-principal authors. For correspondence: BLL (biology), F.J. Kao, P.C. Cheng and M. Gu (optics).",
year = "2001",
month = oct,
doi = "10.1016/S0968-4328(00)00068-8",
language = "English",
volume = "32",
pages = "661--669",
journal = "Micron",
issn = "0968-4328",
publisher = "Elsevier Ltd",
number = "7",
}