摘要
A silicon nanowire field-effect transistor (SiNW-FET) coated with a polyvinyl chloride (PVC) membrane
containing valinomycin (VAL) was employed as a biosensor (referred to as VAL-PVC/SiNW-FET) to detect
the K+-efflux from live chromaffin cells. The detection sensitivity of K+ with the VAL-PVC/SiNW-FET covers a broad range of concentrations from 10−6 to 10−2 M. The apparent association constants between VAL and Li+, Na+, K+, and Cs+ in Tris buffer solution were determined to be 67 ± 42, 120 ± 23, 5974 ± 115, and
4121 ± 140 M−1, respectively. By culturing chromaffin cells on the VAL-PVC/SiNW-FET, the conductance was significantly increased by nicotine stimulation in a bath buffer without Na+. The K+ concentration at the cell surface was determined to be ∼20 M under the stimulation of 5 mM nicotine. These results demonstrate that the VAL-PVC/SiNW-FET is sensitive and selective to detect the released K+ from cells and is suitable for applications in cellular recording investigations.
containing valinomycin (VAL) was employed as a biosensor (referred to as VAL-PVC/SiNW-FET) to detect
the K+-efflux from live chromaffin cells. The detection sensitivity of K+ with the VAL-PVC/SiNW-FET covers a broad range of concentrations from 10−6 to 10−2 M. The apparent association constants between VAL and Li+, Na+, K+, and Cs+ in Tris buffer solution were determined to be 67 ± 42, 120 ± 23, 5974 ± 115, and
4121 ± 140 M−1, respectively. By culturing chromaffin cells on the VAL-PVC/SiNW-FET, the conductance was significantly increased by nicotine stimulation in a bath buffer without Na+. The K+ concentration at the cell surface was determined to be ∼20 M under the stimulation of 5 mM nicotine. These results demonstrate that the VAL-PVC/SiNW-FET is sensitive and selective to detect the released K+ from cells and is suitable for applications in cellular recording investigations.
| 原文 | American English |
|---|---|
| 頁（從 - 到） | 137-143 |
| 期刊 | Biosensors and Bioelectronics |
| 卷 | 31 |
| 出版狀態 | Published - 2012 |