Monitoring cellular metabolism of 3T3 upon wild type E.coli infection by mapping NADH with FLIM

Tatyana Buryakina, Pin Tzu Su, Vladimir Gukassyan, Wan Jr Syu, Fu Jen Kao*

*此作品的通信作者

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

Fluorescence lifetime imaging microscopy (FLIM) has gained popularity as a sensitive technique to monitor the functional/conformational states of reduced nicotinamide adenine dinucleotide (NADH), one of the main compounds of oxidative phosphorylation. In this letter, we apply the technique to characterize the metabolic changes in mouse embryonic fibroblast 3T3 cells upon bacterial infection. A gradual shortening of the decaying time constants in both the short and the long lifetime components of NADH's autofluorescence is detected. The ratio of the short and the long lifetime components' relative contributions, however, shows a rapid increase, indicating the rise of cellular metabolic activity over the course of infection.

原文English
頁(從 - 到)931-933
頁數3
期刊Chinese Optics Letters
8
發行號10
DOIs
出版狀態Published - 10月 2010

指紋

深入研究「Monitoring cellular metabolism of 3T3 upon wild type E.coli infection by mapping NADH with FLIM」主題。共同形成了獨特的指紋。

引用此