Modulating the activity of the channel-forming segment of Vpr protein from HIV-1

Chin Pei Chen, Clemens Kremer, Peter Henklein, Ulrich Schubert, Rainer H.A. Fink, Wolfgang B. Fischer

研究成果: Article同行評審

4 引文 斯高帕斯(Scopus)

摘要

Viral protein of regulation (Vpr) encoded by human immunodeficiency virus type 1 (HIV-1) is a short auxiliary protein that is 96 amino acids in length. During the viral life cycle, Vpr is released into the blood serum and is able to enter cellular membranes of noninfected cells. In this study a short peptide, Vpr55-83, was shown to exhibit ion-channel-like activity when reconstituted into (1) planar lipid bilayers and (2) lipid bilayers held at the tip of a glass pipette. The two set-ups led to differences in the oligomerization state of the peptide, which was rejected in differences in the conductance levels. Experiments under applied hydrostatic pressure affect the dynamics of the protein within the membrane.

原文English
頁(從 - 到)1089-1095
頁數7
期刊European Biophysics Journal
39
發行號7
DOIs
出版狀態Published - 6月 2010

指紋

深入研究「Modulating the activity of the channel-forming segment of Vpr protein from HIV-1」主題。共同形成了獨特的指紋。

引用此