Micropatterning and characterization of electrospun PCL/gelatin nanofiber tissue scaffolds by femtosecond laser ablation

Yong Chae Lim, Jed Johnson, Zhengzheng Fei, Dave F. Farson, John J. Lannutti, Hae Woon Choi, L. James Lee

研究成果: Conference contribution同行評審

摘要

PCL/gelatin is a biodegradable polymer blend with good biocompatibility, mechanical, physical and chemical properties that is a promising material for use in fabrication of electrospun (ES) nanofiber tissue engineering scaffolds. Although ES nanofiber scaffolds provide a favorable extracellular matrix, microscale surface patterns are needed for study and control of cell adhesion, proliferation and migration. However, micro-patterning of ES polymer/biomaterial blended nanofibers with available techniques is difficult due to its fibrous surface texture and biomaterial content. In this work, the effects of direct-write femtosecond laser ablation on electrospun PCL/gelatin nanofiber tissue scaffold surface morphology and chemistry and resulting growth rate of deposited cells was investigated. Scanning electron microscope (SEM) images showed that laser ablation did not change the statistical distributions of fiber diameter and surface porosity. Attenuated Total Reflectance Fourier Transform Infrared (ATR- FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) revealed no changes in the chemical states of PCL/gelatin nanofiber before and after femtosecond laser irradiation. In vitro, mouse embryonic stem (mES) cell initial growth rates were similar on both as-spun surface and micropockets made by femtosecond laser ablation. It was concluded that femtosecond laser processing is effective for creating 3-dimensional microscale patterns on ES nanofiber tissue scaffolds.

原文English
主出版物標題ICALEO 2009 - 28th International Congress on Applications of Lasers and Electro-Optics, Congress Proceedings
發行者Laser Institute of America
頁面1114-1123
頁數10
ISBN(列印)9780912035598
DOIs
出版狀態Published - 2009
事件28th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2009 - Orlando, FL, United States
持續時間: 2 11月 20095 11月 2009

出版系列

名字ICALEO 2009 - 28th International Congress on Applications of Lasers and Electro-Optics, Congress Proceedings
102

Conference

Conference28th International Congress on Applications of Lasers and Electro-Optics, ICALEO 2009
國家/地區United States
城市Orlando, FL
期間2/11/095/11/09

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