Metabolic engineering of 2-pentanone synthesis in Escherichia coli

Ethan I. Lan, Yasumasa Dekishima, Derrick S. Chuang, James C. Liao*

*此作品的通信作者

研究成果: Article同行評審

20 引文 斯高帕斯(Scopus)

摘要

Expanding the chemical diversity of microbial fermentation products enables green production of fuel, chemicals, and pharmaceuticals. In recent years, coenzyme A (CoA) dependent chain elongation, resembling the reversed β-oxidation pathway, has attracted interest for its use in producing higher alcohols, fatty acids, and polyhydroxyalkanoate. To expand the chemical diversity of this pathway, we metabolically engineered Escherichia coli to produce 2-pentanone, which is not a natural fermentation product of E. coli. We describe the first demonstration of 2-pentanone synthesis in E. coli by coupling the CoA-dependent chain elongation with the acetone production pathway. By bioprospecting for enzymes capable of efficient hydrolysis of 3-keto-hexanoyl-CoA, production of 2-pentanone increased 20 fold, reaching a titer of 240 mg/L.

原文English
頁(從 - 到)3167-3175
頁數9
期刊AIChE Journal
59
發行號9
DOIs
出版狀態Published - 9月 2013

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