MEK2 is a critical modulating mechanism to down-regulate GCIP stability and function in cancer cells

Ruei Yue Liang, Bang Hung Liu, Chih Jou Huang, Kuan Ting Lin, Chih Chung Ko, Lin Lun Huang, Bin Hsu, Chun Ying Wu, Show Mei Chuang*

*此作品的通信作者

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

Loss of tumor suppressor activity and upregulation of oncogenic pathways simultaneously contribute to tumorigenesis. Expression of the tumor suppressor, GCIP (Grap2- and cyclin D1-interacting protein), is usually reduced or lost in advanced cancers, as seen in both mouse tumor models and human cancer patients. However, no previous study has examined how cancer cells down-regulate GCIP expression. In this study, we first validate the tumor suppressive function of GCIP using clinical gastric cancer tissues and online database analysis. We then reveal a novel mechanism whereby MEK2 directly interacts with and phosphorylates GCIP at its Ser313 and Ser356 residues to promote the turnover of GCIP by ubiquitin-mediated proteasomal degradation. We also reveal that decreased GCIP stability enhances cell proliferation and promotes cancer cell migration and invasion. Taken together, these findings provide a more comprehensive view of GCIP in tumorigenesis and suggest that the oncogenic MEK/ERK signaling pathway negatively regulates the protein level of GCIP to promote cell proliferation and migration.

原文English
頁(從 - 到)1958-1969
頁數12
期刊FASEB Journal
34
發行號2
DOIs
出版狀態Published - 1 2月 2020

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