@inproceedings{732aa4dbbd41419fb445b2f84031ed11,
title = "Mechanism of two-photon fluorescence increment via crosslinked bovine serum albumin",
abstract = "The two-photon excited fluorescence (TPEF) increments of two dyes via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking technique were investigated. One is Rose Bengal (RB) with a high non-radiative decay rate, while the other is Eosin Y with a low non-radiative decay rate. Experimental results demonstrate that the quantum yield and lifetime of RB are both augmented via crosslinked BSA microstructures. Compared with theoretical analysis, this result indicates that the non-radiative decay rate of RB is decreased; hence, the quenched effect induced by BSA solution is suppressed. However, the fluorescence lifetime of Eosin Y is acutely abated despite the augmented quantum yield for the two-photon crosslinking processing from BSA solution. This result deduces that the radiative decay rate increased. Furthermore, the increased TPEF intensity and lifetime of RB correlated with the concentration of fabricated crosslinked BSA microstructures through pulse selection of the employed femtosecond laser is demonstrated and capable of developing a zone-plate-like BSA microstructure.",
keywords = "Bovine serum albumin, Fluorescence increment, Multiphoton microfabrication, Two-photon",
author = "Lin, {C. Y.} and Lien, {C. H.} and Chang, {C. Y.} and Chen, {S. J.}",
year = "2012",
doi = "10.1117/12.930335",
language = "English",
isbn = "9780819491763",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Physical Chemistry of Interfaces and Nanomaterials XI",
note = "Physical Chemistry of Interfaces and Nanomaterials XI ; Conference date: 12-08-2012 Through 15-08-2012",
}