Measurement of macrophage adhesion by optical tweezers with backward-scattered detection

Sung Yang Wei*, Yi Jr Su, Po Chen Shih, Shih Mo Yang, Long Hsu

*此作品的通信作者

研究成果: Conference contribution同行評審

摘要

Macrophages are members of the leukocyte family. Tissue damage causes inflammation and release of vasoactive and chemotactic factors, which trigger a local increase in blood flow and capillary permeability. Then, leukocytes accumulate quickly to the infection site. The leukocyte extravasation process takes place according to a sequence of events that involve tethering, activation by a chemoattractant stimulus, adhesion by integrin binding, and migrating to the infection site. The leukocyte extravasation process reveals that adhesion is an important part of the immune system. Optical tweezers have become a useful tool with broad applications in biology and physics. In force measurement, the trapped bead as a probe usually uses a polystyrene bead of 1 μm diameter to measure adhesive force between the trapped beads and cell by optical tweezers. In this paper, using the ray-optics model calculated trapping stiffness and defined the linear displacement ranges. By the theoretical values of stiffness and linear displacement ranges, this study attempted to obtain a proper trapped particle size in measuring adhesive force. Finally, this work investigates real-time adhesion force measurements between human macrophages and trapped beads coated with lipopolysaccharides using optical tweezers with backscattered detection.

原文English
主出版物標題Optical Trapping and Optical Micromanipulation VII
DOIs
出版狀態Published - 27 10月 2010
事件Optical Trapping and Optical Micromanipulation VII - San Diego, CA, United States
持續時間: 1 8月 20105 8月 2010

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
7762
ISSN(列印)0277-786X

Conference

ConferenceOptical Trapping and Optical Micromanipulation VII
國家/地區United States
城市San Diego, CA
期間1/08/105/08/10

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