Mcm3 is polyubiquitinated during mitosis before establishment of the pre-replication complex

Irene H. Cheng, Louis A. Roberts, Bik K. Tye*

*此作品的通信作者

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

To ensure fidelity in genome duplication, eukaryotes restrict DNA synthesis to once every cell division by a cascade of regulated steps. Central to this cascade is the periodic assembly of the hexameric MCM2-7 complex at replication origins. However, in Saccharomyces cerevisiae, only a fraction of each MCM protein is able to assemble into hexamers and associate with replication origins during M phase, suggesting that MCM complex assembly and recruitment may be regulated post-translationally. Here we show that a small fraction of Mcm3p is polyubiquitinated at the onset of MCM complex assembly. Reducing the rate of ubiquitination by uba1165, a suppressor of mcm3-10, restored the interaction of Mcm3-10p with subunits of the MCM complex and its recruitment to the replication origin. Possible roles for ubiquitinated Mcm3p in the assembly of the MCM complex at replication origins are discussed.

原文English
頁(從 - 到)41706-41714
頁數9
期刊Journal of Biological Chemistry
277
發行號44
DOIs
出版狀態Published - 1 11月 2002

指紋

深入研究「Mcm3 is polyubiquitinated during mitosis before establishment of the pre-replication complex」主題。共同形成了獨特的指紋。

引用此