摘要
We have demonstrated widely tunable Yb:fiber-based laser sources, aiming to replace Ti:sapphire lasers for the nJ-level ultrafast applications, especially for the uses of nonlinear light microscopy. We investigated the influence of different input parameters to obtain an expansive spectral broadening, enabled by self-phase modulation and further reshaped by self-steepening, in the normal dispersion regime before the fiber damage. We also discussed the compressibility and intensity fluctuations of the demonstrated pulses, to reach the transform-limited duration with a very low intensity noise. Most importantly, we have demonstrated clear two-photon fluorescence images from UV-absorbing fluorophores to deep red dye stains.
原文 | English |
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頁(從 - 到) | 2888-2901 |
頁數 | 14 |
期刊 | Biomedical Optics Express |
卷 | 12 |
發行號 | 5 |
DOIs | |
出版狀態 | Published - 1 5月 2021 |