Line-scanning hyperspectral imaging based on structured illumination optical sectioning

Yu John Hsu, Chih Chiang Chen, Chien Hsiang Huang, Chia Hua Yeh, Li Ying Liu, Szu Yu Chen

研究成果: Article同行評審

9 引文 斯高帕斯(Scopus)

摘要

Line-scanning hyperspectral imaging (LHSI) is known to have a higher acquisition rate but lower sectioning capability than point-scanning hyperspectral imaging. To further increase the axial imaging contrast of LHSI, structured illumination was integrated into line excitation to remove the off-focus and scattered on-focus fluorescence signals. In an unsectioned leaf, the imaging contrast can be enhanced by 8 times, while in sectioned mouse skin tissues, a 4.5-fold enhancement can be achieved. With a spectral resolution of 1.15 nm, the fluorophores with seriously-overlapped spectra was proved to be separated without cross-talk by applying linear unmixing to the recorded spectral information.

原文English
文章編號#282654
期刊Biomedical Optics Express
8
發行號6
DOIs
出版狀態Published - 1 6月 2017

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