Ligand-functionalization of BPEI-coated YVO4:Bi 3+,Eu3+ nanophosphors for tumor-cell-targeted imaging applications

Yi Chin Chen, Sheng Cih Huang, Yu Kuo Wang, Yuan Ting Liu, Tung-Kung Wu, Teng-Ming Chen*


研究成果: Article同行評審

9 引文 斯高帕斯(Scopus)


In this study, surface-functionalized, branched polyethylenimine (BPEI)-modified YVO4:Bi3+,Eu3+ nanocrystals (NCs) were successfully synthesized by a simple, rapid, solvent-free hydrothermal method. The BPEI-coated YVO4:Bi3+,Eu 3+ NCs with high crystallinity show broad-band excitation in the λ=250 to 400 nm near-ultraviolet (NUV) region and exhibit a sharp-line emission band centered at λ=619 nm under excitation at λ=350 nm. The surface amino groups contributed by the capping agent, BPEI, not only improve the dispersibility and water/buffer stability of the BPEI-coated YVO4:Bi3+,Eu3+ NCs, but also provide a capability for specifically targeted biomolecule conjugation. Folic acid (FA) and epidermal growth factor (EGF) were further attached to the BPEI-coated YVO4:Bi3+,Eu3+ NCs and exhibited effective positioning of fluorescent NCs toward the targeted folate receptor overexpressed in HeLa cells or EGFR overexpressed in A431 cells with low cytotoxicity. These results demonstrate that the ligand-functionalized, BPEI-coated YVO 4:Bi3+, Eu3+ NCs show great potential as a new-generation biological luminescent bioprobe for bioimaging applications. Moreover, the unique luminescence properties of BPEI-coated YVO 4:Bi3+,Eu3+ NCs show potential to combine with a UVA photosensitizing drug to produce both detective and therapeutic effects for human skin cancer therapy. Packing a punch! Ligand-functionalized, branched polyethylenimine (BPEI)-coated YVO4:Bi3+,Eu3+ nanocrystals (NCs; ≈20 nm) show a strong red luminescence under near-UV excitation (see picture). In vitro bioimaging results show that ligand-functionalized BPEI-coated YVO4:Bi3+,Eu 3+ NCs can be specifically taken up by cancer cells through a receptor-mediated endocytosis mechanism.

頁(從 - 到)2652-2659
期刊Chemistry - An Asian Journal
出版狀態Published - 1 11月 2013


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