Label-free, multi-contrast optical coherence tomography for study of skin melanoma mice in vivo

Pei Yu Lai, Tim Han Lin, Ya Shuan Chou, Chung Hsing Chang*, Wen Chuan Kuo

*此作品的通信作者

研究成果: Conference contribution同行評審

摘要

The lymphatic system plays an important role in inflammation and cancer such as melanoma. Due to the limitations of current developed imaging techniques, visualization of lymphatic vessels within the tissue in vivo has been challenging. Optical imaging of lymphatic vessel is gaining increased interests because it does not involve any radiation and can achieve very high resolution. Here, we developed a multi-contrast, label-free optical coherence tomography (OCT) imaging technology with an axial resolution of ∼ 5 μm and lateral resolution of ∼ 7 μm, which is capable of providing microstructural information and microcirculatory system including blood and lymphatic vessels simultaneously. Using this technique, we observed the melanoma mice in vivo. Mice were treated topically on the ear with (Z)-4-Hydroxytamoxifen(4-OHT) to elicit BRAFV600E and to silence Pten expression. Also, to observing the structural information, angiogenesis and lymphangiogenesis in the ear of the induced melanoma mouse can be done. The advantage of using OCT over other imaging modalities is its ability to assess label-free blood flow along with lymphatic vessels simultaneously for imaging the microcirculatory system within tissue beds without any exogenous agents. Because the metastasis of melanoma is highly related to the lymphatic vessels, our findings can be a powerful tool to help the diagnosis of the metastasis melanoma. In the future, this may become a helpful tool for better understanding pathologic mechanisms and treatment technique development in some diseases.

原文English
主出版物標題Photonics in Dermatology and Plastic Surgery 2018
編輯Haishan Zeng, Bernard Choi
發行者SPIE
ISBN(電子)9781510614192
DOIs
出版狀態Published - 2018
事件Photonics in Dermatology and Plastic Surgery 2018 - San Francisco, United States
持續時間: 27 1月 201828 1月 2018

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
10467
ISSN(列印)1605-7422

Conference

ConferencePhotonics in Dermatology and Plastic Surgery 2018
國家/地區United States
城市San Francisco
期間27/01/1828/01/18

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