摘要
In this study, we describe a highly sensitive and reusable silicon
nanowire field-effect transistor for the detection of protein-protein
interactions. This reusable device was made possible by the reversible association of glutathione S-transferase-tagged calmodulin with a glutathione modified transistor. The calmodulin-modified transistor exhibited selective electrical responses to Ca2þ (≥1 μM) and purified cardiac troponin I (∼7 nM); the change in conductivity displayed a linear dependence on the concentration of troponin I in a range from 10 nM to 1 μM. These results are consistent with the
previously reported concentration range in which the dissociation constant for the troponin I-calmodulin complex was determined.The minimum concentration of Ca2þ required to activate calmodulin was determined to be 1 μM. We have also successfully demonstrated that the N-type Ca2þ channels, expressed by cultured 293T cells, can be recognized specifically by the calmodulin-modified nanowire transistor. This sensitive nanowire transistor can serve as a high-throughput biosensor and can also substitute for immunoprecipitation methods used in the identification of interacting
proteins.
nanowire field-effect transistor for the detection of protein-protein
interactions. This reusable device was made possible by the reversible association of glutathione S-transferase-tagged calmodulin with a glutathione modified transistor. The calmodulin-modified transistor exhibited selective electrical responses to Ca2þ (≥1 μM) and purified cardiac troponin I (∼7 nM); the change in conductivity displayed a linear dependence on the concentration of troponin I in a range from 10 nM to 1 μM. These results are consistent with the
previously reported concentration range in which the dissociation constant for the troponin I-calmodulin complex was determined.The minimum concentration of Ca2þ required to activate calmodulin was determined to be 1 μM. We have also successfully demonstrated that the N-type Ca2þ channels, expressed by cultured 293T cells, can be recognized specifically by the calmodulin-modified nanowire transistor. This sensitive nanowire transistor can serve as a high-throughput biosensor and can also substitute for immunoprecipitation methods used in the identification of interacting
proteins.
| 原文 | American English |
|---|---|
| 頁（從 - 到） | 1047–1052 |
| 期刊 | Proceedings of the National Academy of Sciences of the United States of America |
| 卷 | 107 |
| 出版狀態 | Published - 2010 |