Kinetics of hydrogen sulfide oxidation by immobilized autotrophic heterotrophic bacteria in bioreactors

The kinetics of H₂S oxidation in bioreactors with separately packed autotrophic Thiobacillus thioparus CH11 and heterotrophic Pseudomonas putida CH11 were evaluated. The reaction rates were determined to be first-order below 20 ppm, zero-order above 60 ppm, and fractional-order in the intermediate concentration ranges for the Thiobacillus thioparus CH11 bioreactor, and first-order below 35 ppm, zero-order above 80 ppm, and fractional-order in the intermediate concentration ranges for the Pseudomonas putida CH11 bioreactor. The saturation constants for H₂S by Thiobacillus thioparus CH11 and Pseudomonas putida CH11 were calculated to be 30.3 ppm and 44.2 ppm, respectively.