In this letter, a junctionless (JL) poly-Si thin-film transistor (TFT) with a 3-nm-thick nanosheet channel is successfully fabricated using the low-temperature atomic level etching process. An inversion-mode (IM) TFT is also prepared for performance comparison and reliability investigation of positive gate bias stress (PGBS). In comparison with the IM-TFT, the JL-TFT exhibits superior PGBS reliability. The origin of the difference in degradation rates between the JL and IM-TFTs is ascribed to the different transport mechanisms and different gate dielectric fields under the same gate over-drive stress. Nanosheet JL-TFTs with a 3-nm channel thickness show excellent S.S (69 mV/decade) and extremely low off-current (1.93 fA). Results indicate that it is a promising candidate for low-power 3-D integrated circuits.