Investigating the effects of plasma pretreatment on the formation of ordered aggregates of lysozyme

Chih Kai Chang; Wei An Chen; Chao Yu Sie; Shen Chieh Lin; Lilian Tsai Wei Lin; Ta Hsien Lin; Cheng Che Hsu; Steven S.S. Wang

doi:10.1016/j.colsurfb.2014.12.017

Investigating the effects of plasma pretreatment on the formation of ordered aggregates of lysozyme

Chih Kai Chang, Wei An Chen, Chao Yu Sie, Shen Chieh Lin, Lilian Tsai Wei Lin, Ta Hsien Lin, Cheng Che Hsu, Steven S.S. Wang^*

^*此作品的通信作者

醫學系生物化學科

研究成果: Article › 同行評審

6 引文斯高帕斯（Scopus）

摘要

We investigated the influence of plasma pretreatment on fibril formation and aggregation properties of lysozyme by using the Congo red binding assay, transmission electron microscopy, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), far-ultraviolet circular dichroism, and 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence spectroscopy. Our Congo red binding and transmission electron microscopy findings indicated that plasma pretreatment may suppress the formation of ordered fibrillar lysozyme aggregates. The inhibitory effect triggered by plasma pretreatment was observed to be positively correlated with the duration of plasma pretreatment. Compared to the untreated controls, our ANS fluorescence results suggested that fewer solvent-exposed hydrophobic clusters in lysozymes were formed upon pretreatment with plasma. Moreover, HEWL samples with and without plasma pretreatment showed considerably different molecular profiles. We believe the outcome from this work may not only help develop potential strategies for the attenuation of ordered protein aggregation, which is implicated in amyloid pathology, but also present a nice example of plasma-based medicine.

原文	English
頁（從 - 到）	154-161
頁數	8
期刊	Colloids and Surfaces B: Biointerfaces
卷	126
發行號	1
DOIs	https://doi.org/10.1016/j.colsurfb.2014.12.017
出版狀態	Published - 1 2月 2015

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title = "Investigating the effects of plasma pretreatment on the formation of ordered aggregates of lysozyme",

abstract = "We investigated the influence of plasma pretreatment on fibril formation and aggregation properties of lysozyme by using the Congo red binding assay, transmission electron microscopy, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), far-ultraviolet circular dichroism, and 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence spectroscopy. Our Congo red binding and transmission electron microscopy findings indicated that plasma pretreatment may suppress the formation of ordered fibrillar lysozyme aggregates. The inhibitory effect triggered by plasma pretreatment was observed to be positively correlated with the duration of plasma pretreatment. Compared to the untreated controls, our ANS fluorescence results suggested that fewer solvent-exposed hydrophobic clusters in lysozymes were formed upon pretreatment with plasma. Moreover, HEWL samples with and without plasma pretreatment showed considerably different molecular profiles. We believe the outcome from this work may not only help develop potential strategies for the attenuation of ordered protein aggregation, which is implicated in amyloid pathology, but also present a nice example of plasma-based medicine.",

keywords = "Aggregation, Amyloid fibrils, Lysozyme, Plasma",

author = "Chang, {Chih Kai} and Chen, {Wei An} and Sie, {Chao Yu} and Lin, {Shen Chieh} and Lin, {Lilian Tsai Wei} and Lin, {Ta Hsien} and Hsu, {Cheng Che} and Wang, {Steven S.S.}",

note = "Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

year = "2015",

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doi = "10.1016/j.colsurfb.2014.12.017",

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TY - JOUR

T1 - Investigating the effects of plasma pretreatment on the formation of ordered aggregates of lysozyme

AU - Chang, Chih Kai

AU - Chen, Wei An

AU - Sie, Chao Yu

AU - Lin, Shen Chieh

AU - Lin, Lilian Tsai Wei

AU - Lin, Ta Hsien

AU - Hsu, Cheng Che

AU - Wang, Steven S.S.

PY - 2015/2/1

Y1 - 2015/2/1

N2 - We investigated the influence of plasma pretreatment on fibril formation and aggregation properties of lysozyme by using the Congo red binding assay, transmission electron microscopy, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), far-ultraviolet circular dichroism, and 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence spectroscopy. Our Congo red binding and transmission electron microscopy findings indicated that plasma pretreatment may suppress the formation of ordered fibrillar lysozyme aggregates. The inhibitory effect triggered by plasma pretreatment was observed to be positively correlated with the duration of plasma pretreatment. Compared to the untreated controls, our ANS fluorescence results suggested that fewer solvent-exposed hydrophobic clusters in lysozymes were formed upon pretreatment with plasma. Moreover, HEWL samples with and without plasma pretreatment showed considerably different molecular profiles. We believe the outcome from this work may not only help develop potential strategies for the attenuation of ordered protein aggregation, which is implicated in amyloid pathology, but also present a nice example of plasma-based medicine.

AB - We investigated the influence of plasma pretreatment on fibril formation and aggregation properties of lysozyme by using the Congo red binding assay, transmission electron microscopy, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), far-ultraviolet circular dichroism, and 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence spectroscopy. Our Congo red binding and transmission electron microscopy findings indicated that plasma pretreatment may suppress the formation of ordered fibrillar lysozyme aggregates. The inhibitory effect triggered by plasma pretreatment was observed to be positively correlated with the duration of plasma pretreatment. Compared to the untreated controls, our ANS fluorescence results suggested that fewer solvent-exposed hydrophobic clusters in lysozymes were formed upon pretreatment with plasma. Moreover, HEWL samples with and without plasma pretreatment showed considerably different molecular profiles. We believe the outcome from this work may not only help develop potential strategies for the attenuation of ordered protein aggregation, which is implicated in amyloid pathology, but also present a nice example of plasma-based medicine.

KW - Aggregation

KW - Amyloid fibrils

KW - Lysozyme

KW - Plasma

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DO - 10.1016/j.colsurfb.2014.12.017

M3 - Article

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AN - SCOPUS:84937204911

SN - 0927-7765

VL - 126

SP - 154

EP - 161

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 1

ER -

Investigating the effects of plasma pretreatment on the formation of ordered aggregates of lysozyme

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