@inbook{c048e4aa49184f17b2df3e9bed19fb3b,
title = "Intestinal crypt organoid: Isolation of intestinal stem cells, in vitro culture, and optical observation",
abstract = "The isolation and culture of intestinal stem cells (ISCs) was first demonstrated in the very recent decade with the identification of ISC marker Lgr5. The growth of ISCs into crypt organoids provides an in vitro model for studying the mucosal physiology, intestinal cancer tumorigenesis, and intestinal regeneration. Here, we describe two different isolation protocols and demonstrate a fixation method that aids in the confocal observation of the organoids.",
keywords = "Confocal observation, Crypt organoid, Intestinal stem cell (ISC), Isolation, Three-dimensional imaging",
author = "Yun Chen and Chuan Li and Tsai, {Ya Hui} and Tseng, {Sheng Hong}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",
year = "2019",
doi = "10.1007/7651_2017_21",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "215--228",
booktitle = "Methods in Molecular Biology",
}
