Intelligent whole-blood imaging flow cytometry for simple, rapid, and cost-effective drug-susceptibility testing of leukemia

Hirofumi Kobayashi, Cheng Lei*, Yi Wu, Chun Jung Huang, Atsushi Yasumoto, Masahiro Jona, Wenxuan Li, Yunzhao Wu, Yaxiaer Yalikun, Yiyue Jiang, Baoshan Guo, Chia-Wei Sun, Yo Tanaka, Makoto Yamada, Yutaka Yatomi, Keisuke Goda

*此作品的通信作者

研究成果: Article同行評審

44 引文 斯高帕斯(Scopus)

摘要

Drug susceptibility (also called chemosensitivity) is an important criterion for developing a therapeutic strategy for various cancer types such as breast cancer and leukemia. Recently, functional assays such as high-content screening together with genomic analysis have been shown to be effective for predicting drug susceptibility, but their clinical applicability is poor since they are time-consuming (several days long), labor-intensive, and costly. Here we present a highly simple, rapid, and cost-effective liquid biopsy for ex vivo drug-susceptibility testing of leukemia. The method is based on an extreme-throughput (>1 million cells per second), label-free, whole-blood imaging flow cytometer with a deep convolutional autoencoder, enabling image-based identification of the drug susceptibility of every single white blood cell in whole blood within 24 hours by simply flowing a drug-treated whole blood sample as little as 500 μL into the imaging flow cytometer without labeling. Our results show that the method accurately evaluates the drug susceptibility of white blood cells from untreated patients with acute lymphoblastic leukemia. Our method holds promise for affordable precision medicine.

原文English
頁(從 - 到)2688-2698
頁數11
期刊Lab on a Chip
19
發行號16
DOIs
出版狀態Published - 21 8月 2019

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