Inhibitory Effect of Excessive Glucose on Its Biochemical Pathway and the Growth of Chinese Hamster Ovary (CHO) Cells

Tsung Yih Lee, Hsiao Hsien Lin, Chien Lung Chen, Shiaw Min Hwang, Ching-Ping Tseng*

*此作品的通信作者

研究成果: Article同行評審

4 引文 斯高帕斯(Scopus)

摘要

In this study, we found the specific growth rate of CHO cells being reduced when glucose increased from 1 to 10 g/L. Under high glucose, we show a 41% increase in lactate dehydrogenase activity consistent with a 70% rate increase in lactate production. The pyruvate kinase activity was significantly increased. However, the activities of citrate synthase, isocitrate dehydrogenase, and malate dehydrogenase involved in the TCA cycle were attenuated. We propose that pyruvate produced from glycolysis is utilized in a lesser extent over the TCA cycle under high-glucose conditions. Thus, the pyruvate is largely converted to lactate, which is toxic to the cells.

原文English
頁(從 - 到)1-11
頁數11
期刊Journal of Carbohydrate Chemistry
34
發行號1
DOIs
出版狀態Published - 2 1月 2015

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