Inhibition of focal contact formation in cells transformed by p 185(neu)

Su Shun Lo, Su Hao Lo, Shao Chun Wang, Mien Chie Hung*


研究成果: Article同行評審

8 引文 斯高帕斯(Scopus)


Signaling pathways mediated by adhesive molecules are tightly associated with cytoskeletal organization and cell growth regulation. Focal adhesion kinase (FAK) plays a prominent role in the adhesion signaling pathway through its tyrosine kinase activity and protein-protein interaction with other signaling molecules, including src, paxillin, and p130(CAS), and other proteins. We explored the roles of these signaling molecules in the transformation of B104-1-1 cells, an NIH/3T3-derived cell line transformed by activated rat p185(neu). The cytoskeletal organization of the p185(neu)- transformed cells was disrupted, and their morphology was dramatically altered. FAK, paxillin, and p130(CAS) appeared to be tyrosine phosphorylated in both NIH/3T3 and B104-1-1. However, the phosphorylation levels of paxillin and p130(CAS) were lower in B104-1-1 cells than in NIH/3T3 cells. Surprisingly, the association between FAK and paxillin was enhanced in B104- 1-1 cells, suggesting reorganization of protein-protein interaction modulated by protein phosphorylation. Our results showed that even though cellular transformation by src and neu has similar consequences, such as focal adhesion disassembly and increased metastasis potential, the molecular events underlying the signaling pathways can be dramatically different.

頁(從 - 到)150-154
期刊Molecular Carcinogenesis
出版狀態Published - 1999


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