In vivo blood lactic acid monitoring using microdialysis and surface-enhanced Raman spectroscopy

Po Hsiang Hsu*, Tung Hu Tsai, Huihua Kenny Chiang


研究成果: Conference contribution同行評審

4 引文 斯高帕斯(Scopus)


Blood lactic acid concentration is an important indicator for physiological functions. To develop a rapid and sensitive measurement technique for monitoring blood lactic acid may provide a useful tool in clinical diagnosis. We proposed to develop a microdialysis surface-enhanced Raman spectroscopy (microdialysis-SERS) approach to filter/reduce interference from other large metabolites in blood and enhance the detection sensitivity for blood lactic acid. In this study, a microdialysis probe was constructed using 13 kDa cut-off dialysis membrane. The dialysate was mixed with 50 nm Ag colloidal nanoparticles automatically in a micro-fluid chamber for SERS detection under blood microdialysis of Sprague-Dawley rat. The linear range of SERS-lactic acid measurement is 10-5∼3x10-4 M with R2 value of 0.99. The optimal mixing flow rate of nanoparticles is 18 μl/min under microdialysis at constant flow rate (2 μl/min). Real time lactic acid monitoring in vivo also has been demonstrated using microdialysis-SERS system.

主出版物子標題Processing, Characterization, and Applications
出版狀態Published - 2008
事件Nanobiosystems: Processing, Characterization, and Applications - San Diego, CA, United States
持續時間: 12 8月 200814 8月 2008


名字Proceedings of SPIE - The International Society for Optical Engineering


ConferenceNanobiosystems: Processing, Characterization, and Applications
國家/地區United States
城市San Diego, CA


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