@inproceedings{82003c819ecc41938a5cacdeca9ae724,
title = "In vivo blood lactic acid monitoring using microdialysis and surface-enhanced Raman spectroscopy",
abstract = "Blood lactic acid concentration is an important indicator for physiological functions. To develop a rapid and sensitive measurement technique for monitoring blood lactic acid may provide a useful tool in clinical diagnosis. We proposed to develop a microdialysis surface-enhanced Raman spectroscopy (microdialysis-SERS) approach to filter/reduce interference from other large metabolites in blood and enhance the detection sensitivity for blood lactic acid. In this study, a microdialysis probe was constructed using 13 kDa cut-off dialysis membrane. The dialysate was mixed with 50 nm Ag colloidal nanoparticles automatically in a micro-fluid chamber for SERS detection under blood microdialysis of Sprague-Dawley rat. The linear range of SERS-lactic acid measurement is 10-5∼3x10-4 M with R2 value of 0.99. The optimal mixing flow rate of nanoparticles is 18 μl/min under microdialysis at constant flow rate (2 μl/min). Real time lactic acid monitoring in vivo also has been demonstrated using microdialysis-SERS system.",
keywords = "13 kDa cut-off dialysis membrane, Ag colloidal nanoparticles, Lactic acid, Microdialysis, Microdialysis probe, Surface-enhanced Raman spectroscopy",
author = "Hsu, {Po Hsiang} and Tsai, {Tung Hu} and Chiang, {Huihua Kenny}",
year = "2008",
doi = "10.1117/12.794798",
language = "English",
isbn = "9780819472601",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Nanobiosystems",
note = "Nanobiosystems: Processing, Characterization, and Applications ; Conference date: 12-08-2008 Through 14-08-2008",
}