In Situ Activation of Pituitary-Infiltrating T Lymphocytes in Autoimmune Hypophysitis

Han Huei Lin, Angelika Gutenberg, Tzu Yu Chen, Nu Man Tsai, Chia Jung Lee, Yu Che Cheng, Wen Hui Cheng, Ywh Min Tzou, Patrizio Caturegli, Shey-Cherng Tzou*


研究成果: Article同行評審

22 引文 斯高帕斯(Scopus)


Autoimmune hypophysitis (AH) is a chronic inflammatory disease characterized by infiltration of T and B lymphocytes in the pituitary gland. The mechanisms through which infiltrating lymphocytes cause disease remain unknown. Using a mouse model of AH we assessed whether T lymphocytes undergo activation in the pituitary gland. Infiltrating T cells co-localized with dendritic cells in the pituitary and produced increased levels of interferon-Î 3 and interleukin-17 upon stimulation in vitro. Assessing proliferation of CD3-and B220-postive lymphocytes by double immunohistochemistry (PCNA-staining) and flow cytometry (BrdU incorporation) revealed that a discrete proportion of infiltrating T cells and B cells underwent proliferation within the pituitary parenchyma. This proliferation persisted into the late disease stage (day 56 post-immunization), indicating the presence of a continuous generation of autoreactive T and B cells within the pituitary gland. T cell proliferation in the pituitary was confirmed in patients affected by autoimmune hypophysitis. In conclusion, we show that pituitary-infiltrating lymphocytes proliferate in situ during AH, providing a previously unknown pathogenic mechanism and new avenues for treatment.

頁(從 - 到)1-10
期刊Scientific reports
出版狀態Published - 6 3月 2017


深入研究「In Situ Activation of Pituitary-Infiltrating T Lymphocytes in Autoimmune Hypophysitis」主題。共同形成了獨特的指紋。