@inproceedings{b8dfbfa9245a4b62b90df093d48c969b,
title = "Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination",
abstract = "A developed temporal focusing-based multiphoton microscope (TFMPM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPM can be condensed from 3.0 μm to 1.5 μm for a 50% improvement. Furthermore, a multiline scanning mechanism based on the DMD can push its AEC nearly equivalent to line scanningbased TFMPM to 1.5 μm from optimal 3.0 μm of the conventional TFMPM. By using the TFMPM with structured illumination and multiline scanning, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression.",
keywords = "Medical and biological imaging, Multiphoton processes, Nonlinear microscopy",
author = "Chang, {Chia Yuan} and Lin, {Chun Yu} and Hovhannisyan, {Vladimir A.} and Chen, {Shean Jen}",
note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; Advances in Microscopic Imaging II 2019 ; Conference date: 26-06-2019 Through 27-06-2019",
year = "2019",
doi = "10.1117/12.2526908",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "OSA - The Optical Society",
editor = "Emmanuel Beaurepaire and Pavone, {Francesco Saverio}",
booktitle = "Advances in Microscopic Imaging II",
address = "United States",
}