TY - JOUR
T1 - Imaging carious dental tissues with multiphoton fluorescence lifetime imaging microscopy
AU - Lin, Po Yen
AU - Lyu, Hong Chou
AU - Hsu, Chin Ying Stephen
AU - Chang, Chia Seng
AU - Kao, Fu Jen
PY - 2011/1/1
Y1 - 2011/1/1
N2 - In this study, multiphoton excitation was utilized to image normal and carious dental tissues noninvasively. Unique structures in dental tissues were identified using the available multimodality (second harmonic, autofluorescence, and fluorescence lifetime analysis) without labeling. The collagen in dentin exhibits a strong second harmonic response. Both dentin and enamel emit strong autofluorescence that reveals in detail morphological features (such as dentinal tubules and enamel rods) and, despite their very similar spectral profiles, can be differentiated by lifetime analysis. Specifically, the carious dental tissue exhibits a greatly reduced autofluorescence lifetime, which result is consistent with the degree of demineralization, determined by micro-computed tomography. Our findings suggest that two-photon excited fluorescence lifetime imaging may be a promising tool for diagnosing and monitoring dental caries.
AB - In this study, multiphoton excitation was utilized to image normal and carious dental tissues noninvasively. Unique structures in dental tissues were identified using the available multimodality (second harmonic, autofluorescence, and fluorescence lifetime analysis) without labeling. The collagen in dentin exhibits a strong second harmonic response. Both dentin and enamel emit strong autofluorescence that reveals in detail morphological features (such as dentinal tubules and enamel rods) and, despite their very similar spectral profiles, can be differentiated by lifetime analysis. Specifically, the carious dental tissue exhibits a greatly reduced autofluorescence lifetime, which result is consistent with the degree of demineralization, determined by micro-computed tomography. Our findings suggest that two-photon excited fluorescence lifetime imaging may be a promising tool for diagnosing and monitoring dental caries.
UR - http://www.scopus.com/inward/record.url?scp=83455179310&partnerID=8YFLogxK
U2 - 10.1364/BOE.2.000149
DO - 10.1364/BOE.2.000149
M3 - Article
AN - SCOPUS:83455179310
SN - 2156-7085
VL - 2
SP - 149
EP - 158
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 1
ER -