Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4

Yu Shan Lin, Kuang Yung Huang, Hui Chun Yu, Ming Chi Lu, Cheng Jhong Fan, Hsien Yu Huang Tseng, Bi Yao Jhuang, Su Qin Liu, Ning Sheng Lai, Ta Hsien Lin, Hsien Bin Huang*

*此作品的通信作者

研究成果: Article同行評審

摘要

Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.

原文English
頁(從 - 到)236-241
頁數6
期刊Biochemical and Biophysical Research Communications
531
發行號2
DOIs
出版狀態Published - 15 10月 2020

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