Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4

Yu Shan Lin; Kuang Yung Huang; Hui Chun Yu; Ming Chi Lu; Cheng Jhong Fan; Hsien Yu Huang Tseng; Bi Yao Jhuang; Su Qin Liu; Ning Sheng Lai; Ta Hsien Lin; Hsien Bin Huang

doi:10.1016/j.bbrc.2020.07.030

Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4

Yu Shan Lin, Kuang Yung Huang, Hui Chun Yu, Ming Chi Lu, Cheng Jhong Fan, Hsien Yu Huang Tseng, Bi Yao Jhuang, Su Qin Liu, Ning Sheng Lai, Ta Hsien Lin, Hsien Bin Huang^*

^*此作品的通信作者

醫學系生物化學科

研究成果: Article › 同行評審

摘要

Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.

原文	English
頁（從 - 到）	236-241
頁數	6
期刊	Biochemical and Biophysical Research Communications
卷	531
發行號	2
DOIs	https://doi.org/10.1016/j.bbrc.2020.07.030
出版狀態	Published - 15 10月 2020

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10.1016/j.bbrc.2020.07.030

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Lin, Y. S., Huang, K. Y., Yu, H. C., Lu, M. C., Fan, C. J., Huang Tseng, H. Y., Jhuang, B. Y., Liu, S. Q., Lai, N. S., Lin, T. H., & Huang, H. B. (2020). Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. Biochemical and Biophysical Research Communications, 531(2), 236-241. https://doi.org/10.1016/j.bbrc.2020.07.030

@article{9b4919bcb75f448eb66a71cd0ef69afb,

title = "Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4",

abstract = "Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.",

keywords = "Dynactin, EHD4, Endocytic recycling, Eps 15 homology domain-containing protein 1 (EHD1), KIAA1949, Phostensin",

author = "Lin, {Yu Shan} and Huang, {Kuang Yung} and Yu, {Hui Chun} and Lu, {Ming Chi} and Fan, {Cheng Jhong} and {Huang Tseng}, {Hsien Yu} and Jhuang, {Bi Yao} and Liu, {Su Qin} and Lai, {Ning Sheng} and Lin, {Ta Hsien} and Huang, {Hsien Bin}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = oct,

day = "15",

doi = "10.1016/j.bbrc.2020.07.030",

language = "English",

volume = "531",

pages = "236--241",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4

AU - Lin, Yu Shan

AU - Huang, Kuang Yung

AU - Yu, Hui Chun

AU - Lu, Ming Chi

AU - Fan, Cheng Jhong

AU - Huang Tseng, Hsien Yu

AU - Jhuang, Bi Yao

AU - Liu, Su Qin

AU - Lai, Ning Sheng

AU - Lin, Ta Hsien

AU - Huang, Hsien Bin

PY - 2020/10/15

Y1 - 2020/10/15

N2 - Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.

AB - Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.

KW - Dynactin

KW - EHD4

KW - Endocytic recycling

KW - Eps 15 homology domain-containing protein 1 (EHD1)

KW - KIAA1949

KW - Phostensin

UR - http://www.scopus.com/inward/record.url?scp=85089356444&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2020.07.030

DO - 10.1016/j.bbrc.2020.07.030

M3 - Article

C2 - 32800345

AN - SCOPUS:85089356444

VL - 531

SP - 236

EP - 241

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4

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