Identification of 5,7,3′,4′-tetramethoxyflavone metabolites in rat urine by the isotope-labeling method and ultrahigh-performance liquid chromatography-electrospray ionization-mass spectrometry

Wen Chien Lu, Jenn Feng Sheen, Lucy Sun Hwang*, Guor Jien Wei

*此作品的通信作者

研究成果: Article同行評審

17 引文 斯高帕斯(Scopus)

摘要

5,7,3′,4′-Tetramethoxyflavone (TMF), one of the major polymethoxyflavones (PMFs) isolated from Kaempferia parviflor, has been reported possessing various bioactivities, including antifungal, antimalarial, antimycobacterial, and anti-inflammatory activities. Although several studies on the TMF have been reported, the information about the metabolism of TMF and the structures of TMF metabolites is still not yet clear. In this study, an isotope-labeling method was developed for the identification of TMF metabolites. Three isotope-labeled TMFs (5,7,3′,4′-tetramethoxy[3′-D 3]flavone, 5,7,3′,4′-tetramethoxy[4′-D 3]flavone, and 5,7,3′,4′-tetramethoxy[5,4′-D 6]flavone) were synthesized and administered to rats. The urine samples were collected, and the main metabolites were monitored by ultrahigh-performance liquid chromatography-electrospray ionization-mass spectrometry. Five TMF metabolites were unambiguously identified as 3′-hydroxy-5,7,4′-trimethoxyflavone, 7-hydroxy-5,3′,4′- trimethoxyflavone sulfate, 7-hydroxy-5,3′,4′-trimethoxyflavone, 4′-hydroxy-5,7,3′-trimethoxyflavone, and 5-hydroxy-7,3′, 4′-trimethoxyflavone.

原文English
頁(從 - 到)8123-8128
頁數6
期刊Journal of Agricultural and Food Chemistry
60
發行號33
DOIs
出版狀態Published - 22 8月 2012

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