Identification and characterization of metallothionein III (growth inhibitory factor) from porcine brain

Chian Feng Chen; Sue Hong Wang; Lih Yuan Lin

doi:10.1016/0305-0491(96)00080-6

Identification and characterization of metallothionein III (growth inhibitory factor) from porcine brain

Chian Feng Chen, Sue Hong Wang, Lih Yuan Lin^*

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20 引文斯高帕斯（Scopus）

摘要

Porcine metallothionein III (MTIII) was isolated from brain tissue by the combination of gel filtration and anion-exchange chromatographies. The identity of MTIII was confirmed by comparing the chromatographic characteristics to other MT isoforms isolated from porcine liver. Porcine MTIII has a low molecular weight and a pl of 4.1. This protein contains both zinc and copper, and the zinc can be replaced by cadmium. Using reverse transcriptase-polymerase chain reaction, a 0.2 kb DNA fragment can be amplified from the porcine brain mRNA. This DNA fragment was demonstrated to contain MTIII coding region after cloning and sequencing. The revealed DNA sequence can be translated into 68 amino acids and shows a common structural characteristic of MTIII from other species. Northern blot analysis indicated that MTIII mRNA is expressed in every specified region of the porcine brain examined here.

原文	English
頁（從 - 到）	27-32
頁數	6
期刊	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
卷	115
發行號	1
DOIs	https://doi.org/10.1016/0305-0491(96)00080-6
出版狀態	Published - 1996

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10.1016/0305-0491(96)00080-6

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title = "Identification and characterization of metallothionein III (growth inhibitory factor) from porcine brain",

abstract = "Porcine metallothionein III (MTIII) was isolated from brain tissue by the combination of gel filtration and anion-exchange chromatographies. The identity of MTIII was confirmed by comparing the chromatographic characteristics to other MT isoforms isolated from porcine liver. Porcine MTIII has a low molecular weight and a pl of 4.1. This protein contains both zinc and copper, and the zinc can be replaced by cadmium. Using reverse transcriptase-polymerase chain reaction, a 0.2 kb DNA fragment can be amplified from the porcine brain mRNA. This DNA fragment was demonstrated to contain MTIII coding region after cloning and sequencing. The revealed DNA sequence can be translated into 68 amino acids and shows a common structural characteristic of MTIII from other species. Northern blot analysis indicated that MTIII mRNA is expressed in every specified region of the porcine brain examined here.",

keywords = "brain, cDNA sequence, characterization, gene expression, growth inhibitory factor, isolation, metallothionein III, porcine",

author = "Chen, {Chian Feng} and Wang, {Sue Hong} and Lin, {Lih Yuan}",

year = "1996",

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T1 - Identification and characterization of metallothionein III (growth inhibitory factor) from porcine brain

AU - Chen, Chian Feng

AU - Wang, Sue Hong

AU - Lin, Lih Yuan

PY - 1996

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N2 - Porcine metallothionein III (MTIII) was isolated from brain tissue by the combination of gel filtration and anion-exchange chromatographies. The identity of MTIII was confirmed by comparing the chromatographic characteristics to other MT isoforms isolated from porcine liver. Porcine MTIII has a low molecular weight and a pl of 4.1. This protein contains both zinc and copper, and the zinc can be replaced by cadmium. Using reverse transcriptase-polymerase chain reaction, a 0.2 kb DNA fragment can be amplified from the porcine brain mRNA. This DNA fragment was demonstrated to contain MTIII coding region after cloning and sequencing. The revealed DNA sequence can be translated into 68 amino acids and shows a common structural characteristic of MTIII from other species. Northern blot analysis indicated that MTIII mRNA is expressed in every specified region of the porcine brain examined here.

AB - Porcine metallothionein III (MTIII) was isolated from brain tissue by the combination of gel filtration and anion-exchange chromatographies. The identity of MTIII was confirmed by comparing the chromatographic characteristics to other MT isoforms isolated from porcine liver. Porcine MTIII has a low molecular weight and a pl of 4.1. This protein contains both zinc and copper, and the zinc can be replaced by cadmium. Using reverse transcriptase-polymerase chain reaction, a 0.2 kb DNA fragment can be amplified from the porcine brain mRNA. This DNA fragment was demonstrated to contain MTIII coding region after cloning and sequencing. The revealed DNA sequence can be translated into 68 amino acids and shows a common structural characteristic of MTIII from other species. Northern blot analysis indicated that MTIII mRNA is expressed in every specified region of the porcine brain examined here.

KW - brain

KW - cDNA sequence

KW - characterization

KW - gene expression

KW - growth inhibitory factor

KW - isolation

KW - metallothionein III

KW - porcine

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JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

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Identification and characterization of metallothionein III (growth inhibitory factor) from porcine brain

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