Human costars family protein abracl modulates actin dynamics and cell migration and associates with tumorigenic growth

Bo Yuan Hsiao, Chia Hsin Chen, Ho Yi Chi, Pei Ru Yen, Ying Zhen Yu, Chia Hsin Lin, Te Ling Pang, Wei Chi Lin, Min Lun Li, Yi Chen Yeh, Teh Ying Chou, Mei Yu Chen*

*此作品的通信作者

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

Regulation of cellular actin dynamics is pivotal in driving cell motility. During cancer development, cells migrate to invade and spread; therefore, dysregulation of actin regulators is of-ten associated with cancer progression. Here we report the role of ABRACL, a human homolog of the Dictyostelium actin regulator Costars, in migration and tumorigenic growth of cancer cells. We found a correlation between ABRACL expression and the migratory ability of cancer cells. Cell staining revealed the colocalization of ABRACL and F-actin signals at the leading edge of migrating cells. Analysis of the relative F-/G-actin contents in cells lacking or overexpressing ABRACL sug-gested that ABRACL promotes cellular actin distribution to the polymerized fraction. Physical interaction between ABRACL and cofilin was supported by immunofluorescence staining and proximity ligation. Additionally, ABRACL hindered cofilin-simulated pyrene F-actin fluorescence decay in vitro, indicating a functional interplay. Lastly, analysis on a colorectal cancer cohort demon-strated that high ABRACL expression was associated with distant metastasis, and further explora-tion showed that depletion of ABRACL expression in colon cancer cells resulted in reduced cell proliferation and tumorigenic growth. Together, results suggest that ABRACL modulates actin dynamics through its interaction with cofilin and thereby regulates cancer cell migration and partici-pates in cancer pathogenesis.

原文English
文章編號2037
頁(從 - 到)1-22
頁數22
期刊International Journal Of Molecular Sciences
22
發行號4
DOIs
出版狀態Published - 2 2月 2021

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