High temporal resolution and polarization resolved fluorescence lifetime measurements through stimulated emission

Khalil Ur Rehman, Subir Das, Yih Fan Chen, Fu Jen Kao

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

We have implemented polarization-resolved fluorescence lifetime measurement through stimulated emission based pump-probe technique, which promises much higher temporal resolution (∼4 ps) than conventional time-correlated single-photon counting (TCSPC). The depolarization of ATTO 647N fluorescent dye is resolved through anisotropy fluorescence lifetime measurements, with variable time delay introduced between the pump and the probe beams. Importantly, the polarization anisotropy measurement and the corresponding rotational correlation time characterization of the fluorescent dye are carried out at various temperatures. We have also demonstrated the need of high temporal resolution via hetero Förster energy transfer (Hetero-FRET) through the interaction between the gold nanorods (GNRs) and the fluorescent dye ATTO 647N. Notably, our results compare highly favorably with conventional TCSPC method, which is rather limited in temporal resolution, for the above characterization. Additionally, this technique is applicable even under ambient light while being very cost-effective and robust.

原文English
文章編號024008
期刊Methods and Applications in Fluorescence
8
發行號2
DOIs
出版狀態Published - 4月 2020

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