High-resolution mapping of chromatin packaging in mouse embryonic stem cells and sperm

Benjamin R. Carone, Jui-Hung Hung, Sarah J. Hainer, Min Te Chou, Dawn M. Carone, Zhiping Weng, Thomas G. Fazzio, Oliver J. Rando*

*此作品的通信作者

研究成果: Article同行評審

142 引文 斯高帕斯(Scopus)

摘要

Mammalian embryonic stem cells (ESCs) and sperm exhibit unusual chromatin packaging that plays important roles in cellular function. Here, we extend a recently developed technique, based on deep paired-end sequencing of lightly digested chromatin, to assess footprints of nucleosomes and other DNA-binding proteins genome-wide in murine ESCs and sperm. In ESCs, we recover well-characterized features of chromatin such as promoter nucleosome depletion and further identify widespread footprints of sequence-specific DNA-binding proteins such as CTCF, which we validate in knockdown studies. We document global differences in nuclease accessibility between ESCs and sperm, finding that the majority of histone retention in sperm preferentially occurs in large gene-poor genomic regions, with only a small subset of nucleosomes being retained over promoters of developmental regulators. Finally, we describe evidence that CTCF remains associated with the genome in mature sperm, where it could play a role in organizing the sperm genome.

原文English
頁(從 - 到)11-22
頁數12
期刊Developmental Cell
30
發行號1
DOIs
出版狀態Published - 14 七月 2014

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