High-pressure liquid chromatographic separation of a mixture of corticosteroids, androgens, and progestins

Fore Lien Huang, Ferng Chun Ke, Jiuan Jiuan Hwang, Tung Bin Lo*

*此作品的通信作者

研究成果: Article同行評審

21 引文 斯高帕斯(Scopus)

摘要

Fifteen steroids including corticosteroids, androgens, progestins, and their derivatives were completely separated by reverse-phase high-pressure liquid chromatography on a ChemcoPak 7 ODS-H column in 50 min. The elution procedures were first with water:methanol:acetonitrile:isopropanol 55:32:6.5:7.5 ( v v) for 15 min and followed with a linear gradient elution for 35 min from 0 to 80% of water:methanol:n-butanol 40:40:20 ( v v). The applicability of this method was successfully demonstrated in the analyses of the biological samples of carp plasma, testis, and head kidney.

原文English
頁(從 - 到)512-517
頁數6
期刊Archives of Biochemistry and Biophysics
225
發行號2
DOIs
出版狀態Published - 9月 1983

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