Growth inhibitory effect of the human NIT2 gene and its allelic imbalance in cancers

Chun Hung Lin, Ming Yi Chung, Wen Bin Chen, Chin Hsiang Chien*

*此作品的通信作者

研究成果: Article同行評審

41 引文 斯高帕斯(Scopus)

摘要

The mammalian nitrilase (Nit) protein is a member of the nitrilase superfamily whose function remains to be characterized. We now show that the nitrilase family member 2 gene (NIT2) is ubiquitously expressed in multiple tissues and encodes protein mainly distributed in the cytosol. Ectopic expression of Nit2 in HeLa cells was found to inhibit cell growth through G 2 arrest rather than by apoptosis. Consistent with this, proteomic and RT-PCR analyses showed that Nit2 up-regulated the protein and mRNA levels of 14-3-3σ, an inhibitor of both G2/M progression and protein kinase B (Akt)-activated cell growth, and down-regulated 14-3-3β, a potential oncogenic protein. Genotype analysis in four types of primary tumor tissues showed 12.5-38.5% allelic imbalance surrounding the NIT2 locus. The results demonstrated that NIT2 plays an important role in cell growth inhibition and links to human malignancies, suggesting that Nit2 may be a potential tumor suppressor candidate.

原文English
頁(從 - 到)2946-2956
頁數11
期刊FEBS Journal
274
發行號11
DOIs
出版狀態Published - 6月 2007

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