Green laser excited surface plasmon resonance biosensor utilizing highly sensitive phase interrogation detection

How Foo Chen*, Wei Chen Hsu, Ya Jung Wang, Ta Jen Yen

*此作品的通信作者

研究成果: Conference contribution同行評審

1 引文 斯高帕斯(Scopus)

摘要

Surface plasmon resonance (SPR) biosensors have been widely used for dynamical analysis of molecular affinity, bacterium screening, and drug discovery due to its advantages of label-free detection, dynamic interaction analysis, small sample volume, and ultra sensitivity (feasibility of single molecular detection). Recently, SPR biosensing for cell imaging known as SPR microscopy (SPRM) has attracted great attention due to the characteristics of SPR biosensors. However, it is well known that the trends of sensitivity and spatial resolution are opposite to each other: Surface plasmon waves (SPWs) with shorter wavelength which provides higher spatial resolution has less sensitivity. It is known that the spatial resolution of SPRM is limited by the propagation length of surface plasmon wave (SPW) along the metal-dielectric interface. SPW excited by 632.8 nm light has the propagation length of 3 um. This length becomes longer when a longer wavelength is selected. While most of SPR biosensors are built with 632.8 nm or longer wavelength for high sensitivity, using 532nm light to excite SPWs is desired for submicron resolution since the propagation length is around 150 nm. Different from current phase interrogation methods, the proposed phase interrogation method is highly sensitive and suitable for CCD imaging. Although it is generally believed that SPWs with wavelength 532nm has poor sensitivity, the experimental result showed that the setup can reach the sensitivity lower than 2 ×10-6 RIU when sucrose is used as the test sample.

原文English
主出版物標題Biophotonics
主出版物子標題Photonic Solutions for Better Health Care II
DOIs
出版狀態Published - 2010
事件Biophotonics: Photonic Solutions for Better Health Care II - Brussels, Belgium
持續時間: 12 4月 201016 4月 2010

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
7715
ISSN(列印)1605-7422

Conference

ConferenceBiophotonics: Photonic Solutions for Better Health Care II
國家/地區Belgium
城市Brussels
期間12/04/1016/04/10

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