Genome sequencing and comparative analysis of Klebsiella pneumoniae NTUH-K2044, a strain causing liver abscess and meningitis

Keh Ming Wu, Ng Hui Li, Jing Jou Yan, Nina Tsao, Tsai Lien Liao, Hui Chi Tsai, Chang Phone Fung, Hsiang Ju Chen, Yen Ming Liu, Jin Tung Wang, Chi Tai Fang, Shan Chwen Chang, Hung Yu Shu, Tze Tze Liu, Ying Tsong Chen, Yih Ru Shiau, Tsai Ling Lauderdale, Ih Jen Su, Ralph Kirby*, Shih Feng Tsai

*此作品的通信作者

研究成果: Article同行評審

283 引文 斯高帕斯(Scopus)

摘要

Nosocomial infections caused by antibiotic-resistant Klebsiella pneumoniae are emerging as a major health problem worldwide, while community-acquired K. pneumoniae infections present with a range of diverse clinical pictures in different geographic areas. In particular, an invasive form of K. pneumoniae that causes liver abscesses was first observed in Asia and then was found worldwide. We are interested in how differences in gene content of the same species result in different diseases. Thus, we sequenced the whole genome of K. pneumoniae NTUH-K2044, which was isolated from a patient with liver abscess and meningitis, and analyzed differences compared to strain MGH 78578, which was isolated from a patient with pneumonia. Six major types of differences were found in gene clusters that included an integrative and conjugative element, clusters involved in citrate fermentation, lipopolysaccharide synthesis, and capsular polysaccharide synthesis, phage-related insertions, and a cluster containing fimbria-related genes. We also conducted comparative genomic hybridization with 15 K. pneumoniae isolates obtained from community-acquired or nosocomial infections using tiling probes for the NTUH-K2044 genome. Hierarchical clustering revealed three major groups of genomic insertion-deletion patterns that correlate with the strains' clinical features, antimicrobial susceptibilities, and virulence phenotypes with mice. Here we report the whole-genome sequence of K. pneumoniae NTUH-K2044 and describe evidence showing significant genomic diversity and sequence acquisition among K. pneumoniae pathogenic strains. Our findings support the hypothesis that these factors are responsible for the changes that have occurred in the disease profile over time.

原文English
頁(從 - 到)4492-4501
頁數10
期刊Journal of Bacteriology
191
發行號14
DOIs
出版狀態Published - 7月 2009

指紋

深入研究「Genome sequencing and comparative analysis of Klebsiella pneumoniae NTUH-K2044, a strain causing liver abscess and meningitis」主題。共同形成了獨特的指紋。

引用此