Gene expression profiling and pathway network analysis predicts a novel antitumor function for a botanical-derived drug, PG2

Yu Lun Kuo, Chun Houh Chen, Tsung Hsien Chuang, Wei Kai Hua, Wey-Jinq Lin, Wei Hsiang Hsu, Peter Mu Hsin Chang, Shih Lan Hsu, Tse Hung Huang, Cheng Yan Kao*, Chi Ying F. Huang

*此作品的通信作者

研究成果: Article同行評審

18 引文 斯高帕斯(Scopus)

摘要

PG2 is a botanical drug that is mostly composed of Astragalus polysaccharides (APS). Its role in hematopoiesis and relieving cancer-related fatigue has recently been clinically investigated in cancer patients. However, systematic analyses of its functions are still limited. The aim of this study was to use microarray-based expression profiling to evaluate the quality and consistency of PG2 from three different product batches and to study biological mechanisms of PG2. An integrative molecular analysis approach has been designed to examine significant PG2-induced signatures in HL-60 leukemia cells. A quantitative analysis of gene expression signatures was conducted for PG2 by hierarchical clustering of correlation coefficients. The results showed that PG2 product batches were consistent and of high quality. These batches were also functionally equivalent to each other with regard to how they modulated the immune and hematopoietic systems. Within the PG2 signature, there were five genes associated with doxorubicin: IL-8, MDM4, BCL2, PRODH2, and BIRC5. Moreover, the combination of PG2 and doxorubicin had a synergistic effect on induced cell death in HL-60 cells. Together with the bioinformatics-based approach, gene expression profiling provided a quantitative measurement for the quality and consistency of herbal medicines and revealed new roles (e.g., immune modulation) for PG2 in cancer treatment.

原文English
文章編號917345
期刊Evidence-based Complementary and Alternative Medicine
2015
DOIs
出版狀態Published - 2015

指紋

深入研究「Gene expression profiling and pathway network analysis predicts a novel antitumor function for a botanical-derived drug, PG2」主題。共同形成了獨特的指紋。

引用此